cyclin B1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

cyclin B1 Monoclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1F3G11

Cat no : 67686-1-PBS

Synonyms

CCNB, CCNB1, Cyclin B, cyclin B1, G2/mitotic specific cyclin B1

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Validation Data Gallery

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  WB analysis using 67686-1-Ig (same clone as 67686-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67686-1-Ig (cyclin B1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67686-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67686-1-Ig (same clone as 67686-1-PBS)

  WB result of cyclin B1 antibody (67686-1-Ig; 1:3200; incubated at room temperature for 1.5 hours) with sh-Control and sh-cyclin B1 transfected HeLa cells. This data was developed using the same antibody clone with 67686-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 67686-1-Ig (same clone as 67686-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67686-1-Ig (cyclin B1 antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67686-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 67686-1-Ig (same clone as 67686-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67686-1-Ig (cyclin B1 antibody) at dilution of 1:1600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67686-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67686-1-Ig (same clone as 67686-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using cyclin B1 antibody (67686-1-Ig, Clone: 1F3G11 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67686-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67686-1-Ig (same clone as 67686-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using cyclin B1 antibody (67686-1-Ig, Clone: 1F3G11 ) at dilution of 1:650 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67686-1-PBS in a different storage buffer formulation.

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  IF Staining of HT-29 using 67686-1-Ig (same clone as 67686-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using cyclin B1 antibody (67686-1-Ig, Clone: 1F3G11 ) at dilution of 1:650 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67686-1-PBS in a different storage buffer formulation.

• 

  FC experiment of Jurkat using 67686-1-Ig (same clone as 67686-1-PBS)

  1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human cyclin B1 (67686-1-Ig, Clone:1F3G11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (black). Cells were fixed with 90% MeOH . This data was developed using the same antibody clone with 67686-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67686-1-PBS targets cyclin B1 in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: cyclin B1 fusion protein Ag29444
• Full Name: cyclin B1
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC006510
• Gene Symbol: CCNB1
• Gene ID (NCBI): 891
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -20°C. Stable for one year after shipment.

Background Information

Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase of the cell cycle. The different transcripts result from the use of alternate transcription initiation sites. The antibody is specific to CCNB1. We got a 55-60 kDa band in western blotting maybe due to phosphorylation.