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cyclin B1 Monoclonal antibody

cyclin B1 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1F3G11

Cat no : 67686-1-Ig

Synonyms

CCNB, CCNB1, Cyclin B, cyclin B1, G2/mitotic specific cyclin B1



Tested Applications

Positive WB detected inA549 cells, HeLa cells, NIH/3T3 cells, HSC-T6 cells, K-562 cells, Jurkat cells, HEK-293 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HT-29 cells
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:400-1:1600
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67686-1-Ig targets cyclin B1 in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen cyclin B1 fusion protein Ag29444
Full Name cyclin B1
Calculated Molecular Weight 48 kDa
Observed Molecular Weight55 kDa
GenBank Accession NumberBC006510
Gene Symbol CCNB1
Gene ID (NCBI) 891
RRIDAB_2882879
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase of the cell cycle. The different transcripts result from the use of alternate transcription initiation sites. The antibody is specific to CCNB1. We got a 55-60 kDa band in western blotting maybe due to phosphorylation.

Protocols

Product Specific Protocols
WB protocol for cyclin B1 antibody 67686-1-IgDownload protocol
IHC protocol for cyclin B1 antibody 67686-1-IgDownload protocol
IF protocol for cyclin B1 antibody 67686-1-IgDownload protocol
FC protocol for cyclin B1 antibody 67686-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Adv Sci (Weinh)

Identification of PRDX5 as A Target for The Treatment of Castration-Resistant Prostate Cancer

Authors - Rong Wang
humanWB

Ecotoxicol Environ Saf

The role of ER stress and ATP/AMPK in oxidative stress meditated hepatotoxicity induced by citrinin.

Authors - Jing Wu
human,mouseWB,IF

Cell Signal

MicroRNA-30c-5p arrests bladder cancer G2/M phase and suppresses its progression by targeting PRC1-mediated blocking of CDK1/Cyclin B1 axis

Authors - Yining Hao
humanWB

Front Mol Biosci

Over-Expression and Prognostic Significance of FATP5, as a New Biomarker, in Colorectal Carcinoma.

Authors - Qi-Shun Geng
humanWB

Steroids

The location of estrogen receptor variant ER-α36 is associated with the invasion of glioblastoma

Authors - Hongyan Li
humanWB

Cell Biol Int

Estrogen receptor variant ER-α36 facilitates estrogen signaling via EGFR in glioblastoma

Authors - Chao Qu