luciferase Monoclonal antibody

luciferase Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Gaussia princeps and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

2D2E1

Cat no : 67293-1-Ig

Synonyms

Luciferase, redLUC

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Validation Data Gallery

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  WB analysis of ag13000 using 67293-1-Ig

  Luciferase tagged protein were subjected to SDS PAGE followed by western blot with 67293-1-Ig (Luciferase antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Recombinant protein

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

67293-1-Ig targets luciferase in WB, ELISA applications and shows reactivity with Gaussia princeps samples.

• Tested Reactivity: Gaussia princeps
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: luciferase fusion protein Ag13000
• Full Name: luciferase
• Calculated Molecular Weight: 20 kDa
• GenBank Accession Number: AY015993
• RRID: AB_2882558
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This antibody is designed to detect the luciferase of luciferase (Gaussia princeps) tagged protein.

Publications

Species	Application	Title
human	WB	J Virol; BST2 suppresses LINE-1 retrotransposition by reducing the promoter activity of LINE-1 5'-UTR.; Authors - Yifei Zhao
human	WB	BMC Biotechnol; αvβ3-targeted sEVs for efficient intracellular delivery of proteins using MFG-E8.; Authors - Junxin Mai