Tested Applications
| Positive WB detected in | Recombinant protein |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67293-1-Ig targets luciferase in WB, ELISA applications and shows reactivity with Gaussia princeps samples.
| Tested Reactivity | Gaussia princeps |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13000 Product name: Recombinant dNGLUC protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-185 aa of AY015993 Sequence: MGVKVLFALICIAVAEAKPTENNEDFNIVAVASNFATTDLDADRGKLPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESAQGGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQRCATFASKIQGQVDKIKGAGGD Predict reactive species |
| Full Name | luciferase |
| Calculated Molecular Weight | 20 kDa |
| GenBank Accession Number | AY015993 |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2882558 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
This antibody is designed to detect the luciferase of luciferase (Gaussia princeps) tagged protein.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for luciferase antibody 67293-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The luciferase Monoclonal antibody (2D2E1) (Cat# 67293-1-Ig) has 3 citations published in Scientific Reports, BMC Biotechnology, and Journal of Virology. The associated research fields include heterologous protein expression and signal peptide engineering, extracellular vesicle-mediated intracellular protein delivery, and retrotransposition regulation in virology.
| Species | Application | Title |
|---|---|---|
Sci Rep Identification of improved signal peptides for heterologous expression in Saccharomyces using a screen that exploits Gaussia luciferase | ||
BMC Biotechnol αvβ3-targeted sEVs for efficient intracellular delivery of proteins using MFG-E8. | ||
J Virol BST2 suppresses LINE-1 retrotransposition by reducing the promoter activity of LINE-1 5'-UTR. |
