dsRNA Recombinant antibody

dsRNA Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Dot Blot, ELISA
Cat No. 85780-2-RR
Clone No.250708A2

Host / Isotype

Rabbit / IgG

Reactivity

n/a

Applications

IF/ICC, FC (Intra), Dot Blot, ELISA

250708A2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite®488
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive IF/ICC detected inLive vaccines of TGEV and PEDV-infected Vero cells, Transfected Vero
Positive FC (Intra) detected inTransfected Vero
Positive Dot Blot detected inN/A, Poly(I:C)

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
DOT BLOTDOT BLOT : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85780-2-RR targets dsRNA in IF/ICC, FC (Intra), Dot Blot, ELISA applications and shows reactivity with n/a samples.

Tested Reactivity n/a
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide Predict reactive species
Full Name dsRNA
Gene Symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

dsRNA is formed by the base pairing of two RNA strands, creating a double helix structure similar to DNA, but with some differences. dsRNA can be generated through various mechanisms. In the context of viral infections, it can be derived from the genomes of dsRNA viruses, replication intermediates of single-stranded RNA viruses, or bidirectional transcription of certain DNA viruses. Additionally, aberrant splicing or transcriptional processes may lead to the formation of dsRNA structures. dsRNA is recognized as a pathogen-associated molecular pattern (PAMP) by the immune system. It can trigger a series of immune responses in cells, including the activation of antiviral innate immune pathways.

Protocols

Product Specific Protocols
IF protocol for dsRNA antibody 85780-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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