dsRNA Recombinant antibody

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  IF Staining of Vero using 85780-2-RR

  Immunofluorescent analysis of (4% PFA) fixed Live vaccines of TGEV and PEDV-infected Vero cells using dsRNA antibody (85780-2-RR, Clone: 250708A2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of Transfected Vero using 85780-2-RR

  Immunofluorescent analysis of (4% PFA) fixed Transfected Vero using dsRNA antibody (85780-2-RR, Clone: 250708A2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of Transfected Vero using 85780-2-RR

  1x10^6 Transfected Vero were intracellularly stained with 0.25 ug dsRNA Recombinant antibody (85780-2-RR, Clone:250708A2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), and 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Dot Blot experiment using 85780-2-RR

  Dot blot analysis was used to confirm the specificity of 85780-2-RR anti-dsRNA antibody. samples were spotted onto NC and probed with antibody at 5 µg/ml.The amount of sample (μg) spotted is indicated next to each row.

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  Dot Blot experiment of Poly(I:C) using 85780-2-RR

  Dot blot analysis was used to confirm the specificity of 85780-2-RR dsRNA antibody. samples were spotted onto NC and probed with antibody at 1 µg/ml.The amount of sample (μg/mL) spotted is indicated next to each row.

dsRNA Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra), Dot Blot, ELISA

Cat No. 85780-2-RR

Clone No.250708A2

Host / Isotype

Rabbit / IgG

Reactivity

n/a

Applications

IF/ICC, FC (Intra), Dot Blot, ELISA

Synonyms

250708A2

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Tested Applications

• Positive IF/ICC detected in: Live vaccines of TGEV and PEDV-infected Vero cells, Transfected Vero
• Positive FC (Intra) detected in: Transfected Vero
• Positive Dot Blot detected in: N/A, Poly(I:C)

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
• DOT BLOT: DOT BLOT : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85780-2-RR targets dsRNA in IF/ICC, FC (Intra), Dot Blot, ELISA applications and shows reactivity with n/a samples.

• Tested Reactivity: n/a
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: dsRNA
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

dsRNA is formed by the base pairing of two RNA strands, creating a double helix structure similar to DNA, but with some differences. dsRNA can be generated through various mechanisms. In the context of viral infections, it can be derived from the genomes of dsRNA viruses, replication intermediates of single-stranded RNA viruses, or bidirectional transcription of certain DNA viruses. Additionally, aberrant splicing or transcriptional processes may lead to the formation of dsRNA structures. dsRNA is recognized as a pathogen-associated molecular pattern (PAMP) by the immune system. It can trigger a series of immune responses in cells, including the activation of antiviral innate immune pathways.