Tested Applications
| Positive WB detected in | human plasma, human blood, rabbit serum |
| Positive IHC detected in | human testis tissue, rat testis tissue, rat pancreas tissue, mouse pancreas tissue, rat liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human liver cancer tissue |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:4000-1:16000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67002-1-Ig targets fetuin-B in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.
| Tested Reactivity | human, mouse, rat, rabbit |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12463 Product name: Recombinant human fetuin-B protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 23-382 aa of BC074734 Sequence: QLALNPSALLSRGCNDSDVLAVAGFALRDINKDRKDGYVLRLNRVNDAQEYRRGGLGSLFYLTLDVLETDCHVLRKKAWQDCGMRIFFESVYGQCKAIFYMNNPSRVLYLAAYNCTLRPVSKKKIYMTCPDCPSSIPTDSSNHQVLEAATESLAKYKNENTSKQYSLFKVTRASSQWVVGPSYFVEYLIKESPCTKSQASSCSLQSSDSVPVGLCKGSLTRTHWEKFVSVTCDFFESQAPATGSENSAVNQKPTNLPKVEESQQKNTPPTDSPSKAGPRGSVQYLPDLDDKNSQEKGPQEAFPVHLDLTTNPQGETLDISFLFLEPMEEKLVVLPFPKEKARTAECPGPAQNASPLVLPP Predict reactive species |
| Full Name | fetuin B |
| Calculated Molecular Weight | 382 aa, 42 kDa |
| Observed Molecular Weight | 50 kDa |
| GenBank Accession Number | BC074734 |
| Gene Symbol | Fetuin B |
| Gene ID (NCBI) | 26998 |
| RRID | AB_2882319 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9UGM5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Fetuins A and B are both members of the cystatin superfamily. They are mainly expressed in the liver (hepatokines), as well as other tissues such as the kidneys, tongue, and placenta. Fetuin-B has been identified as a novel adipokine/hepatokine that significantly increases hepatic steatosis and mediates impaired insulin action and glucose intolerance. (PMID: 36915725, PMID: 35702137)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for fetuin-B antibody 67002-1-Ig | Download protocol |
| IHC protocol for fetuin-B antibody 67002-1-Ig | Download protocol |
| WB protocol for fetuin-B antibody 67002-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The fetuin-B Monoclonal antibody (2H7D11), catalog number 67002-1-Ig, has 4 total citations. These appear in four journals: Journal of Agricultural and Food Chemistry, Molecular & Cellular Proteomics, Frontiers in Pharmacology, and Journal of Proteome Research. The associated research fields include nonalcoholic fatty liver disease and de novo lipogenesis, folliculogenesis and infertility, renal nephrotoxicity, and retinal neovascularization, with applications in Western blotting and immunohistochemistry across human and mouse species.
| Species | Application | Title |
|---|---|---|
J Agric Food Chem Baicalein Enhances the Effect of Acarbose on the Improvement of Nonalcoholic Fatty Liver Disease Associated with Prediabetes via the Inhibition of De Novo Lipogenesis. | ||
Mol Cell Proteomics Proteomic analysis of human follicular fluid-derived exosomes reveals that insufficient folliculogenesis in aging women is associated with infertility | ||
Front Pharmacol Delineation of renal protein profiles in aristolochic acid I-induced nephrotoxicity in mice by label-free quantitative proteomics | ||
