fetuin-B Monoclonal antibody

fetuin-B Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, rabbit and More (1)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

3

CloneNo.

2H7D11

Cat no : 67002-1-Ig

Synonyms

FETUB, Fetuin-like protein IRL685, FetuinB, fetuin B, 2H7D11

---

Validation Data Gallery

• 

  WB analysis of human blood using 67002-1-Ig

  human blood were subjected to SDS PAGE followed by western blot with 67002-1-Ig (fetuin-B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human plasma using 67002-1-Ig

  human plasma were subjected to SDS PAGE followed by western blot with 67002-1-Ig (fetuin-B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rabbit serum using 67002-1-Ig

  rabbit serum were subjected to SDS PAGE followed by western blot with 67002-1-Ig (fetuin-B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 67002-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67002-1-Ig (fetuin-B antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 67002-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67002-1-Ig (fetuin-B antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human liver cancer using 67002-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using fetuin-B antibody (67002-1-Ig, Clone: 2H7D11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Tested Applications

• Positive WB detected in: human plasma, human blood, rabbit serum
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human liver cancer tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:150-1:600
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

67002-1-Ig targets fetuin-B in WB, IHC, IF-P, ELISA applications and shows reactivity with human, rabbit samples.

• Tested Reactivity: human, rabbit
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: fetuin-B fusion protein Ag12463
• Full Name: fetuin B
• Calculated Molecular Weight: 382 aa, 42 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC074734
• Gene Symbol: Fetuin B
• Gene ID (NCBI): 26998
• RRID: AB_2882319
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fetuins A and B are both members of the cystatin superfamily. They are mainly expressed in the liver (hepatokines), as well as other tissues such as the kidneys, tongue, and placenta. Fetuin-B has been identified as a novel adipokine/hepatokine that significantly increases hepatic steatosis and mediates impaired insulin action and glucose intolerance. (PMID: 36915725, PMID: 35702137)

Publications

Species	Application	Title
human,mouse	WB	J Agric Food Chem; Baicalein Enhances the Effect of Acarbose on the Improvement of Nonalcoholic Fatty Liver Disease Associated with Prediabetes via the Inhibition of De Novo Lipogenesis.; Authors - Yan Xing
mouse	WB	J Proteome Res; Novel Regulators of Retina Neovascularization: A Proteomics Approach.; Authors - Manhong Xu
mouse	WB	Front Pharmacol; Delineation of renal protein profiles in aristolochic acid I-induced nephrotoxicity in mice by label-free quantitative proteomics; Authors - Xinhui Liu