CoraLite® Plus 488-conjugated fetuin-B Monoclonal antibody

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  IF Staining of HepG2 using CL488-67002

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 fetuin-B antibody (CL488-67002, Clone: 2H7D11 ) at dilution of 1:200, CL594-Phalloidin (red).

fetuin-B Monoclonal Antibody for IF/ICC

Cat No. CL488-67002

Clone No.2H7D11

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

IF/ICC

Synonyms

FETUB, Fetuin-like protein IRL685, FetuinB, fetuin B, 2H7D11

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67002 targets fetuin-B in IF/ICC applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12463

  Product name: Recombinant human fetuin-B protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 23-382 aa of BC074734

  Sequence: QLALNPSALLSRGCNDSDVLAVAGFALRDINKDRKDGYVLRLNRVNDAQEYRRGGLGSLFYLTLDVLETDCHVLRKKAWQDCGMRIFFESVYGQCKAIFYMNNPSRVLYLAAYNCTLRPVSKKKIYMTCPDCPSSIPTDSSNHQVLEAATESLAKYKNENTSKQYSLFKVTRASSQWVVGPSYFVEYLIKESPCTKSQASSCSLQSSDSVPVGLCKGSLTRTHWEKFVSVTCDFFESQAPATGSENSAVNQKPTNLPKVEESQQKNTPPTDSPSKAGPRGSVQYLPDLDDKNSQEKGPQEAFPVHLDLTTNPQGETLDISFLFLEPMEEKLVVLPFPKEKARTAECPGPAQNASPLVLPP

• Full Name: fetuin B
• Calculated Molecular Weight: 382 aa, 42 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC074734
• Gene Symbol: Fetuin B
• Gene ID (NCBI): 26998
• RRID: AB_2919403
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UGM5
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Fetuins A and B are both members of the cystatin superfamily. They are mainly expressed in the liver (hepatokines), as well as other tissues such as the kidneys, tongue, and placenta. Fetuin-B has been identified as a novel adipokine/hepatokine that significantly increases hepatic steatosis and mediates impaired insulin action and glucose intolerance. (PMID: 36915725, PMID: 35702137)