fetuin-B Monoclonal antibody, PBS Only

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  WB analysis of human blood using 67002-1-Ig (same clone as 67002-1-PBS)

  human blood were subjected to SDS PAGE followed by western blot with 67002-1-Ig (fetuin-B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67002-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human plasma using 67002-1-Ig (same clone as 67002-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 67002-1-Ig (fetuin-B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67002-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rabbit serum using 67002-1-Ig (same clone as 67002-1-PBS)

  rabbit serum were subjected to SDS PAGE followed by western blot with 67002-1-Ig (fetuin-B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67002-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 67002-1-Ig (same clone as 67002-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67002-1-Ig (fetuin-B antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67002-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 67002-1-Ig (same clone as 67002-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67002-1-Ig (fetuin-B antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67002-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver cancer using 67002-1-Ig (same clone as 67002-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using fetuin-B antibody (67002-1-Ig, Clone: 2H7D11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67002-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 67002-1-Ig (same clone as 67002-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using fetuin-B antibody (67002-1-Ig, Clone: 2H7D11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67002-1-PBS in a different storage buffer formulation.

fetuin-B Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Cat No. 67002-1-PBS

Clone No.2H7D11

Host / Isotype

Mouse / IgG2b

Reactivity

human, rabbit

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

FETUB, Fetuin-like protein IRL685, FetuinB, fetuin B, 2H7D11

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Product Information

67002-1-PBS targets fetuin-B in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, rabbit samples.

• Tested Reactivity: human, rabbit
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12463

  Product name: Recombinant human fetuin-B protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 23-382 aa of BC074734

  Sequence: QLALNPSALLSRGCNDSDVLAVAGFALRDINKDRKDGYVLRLNRVNDAQEYRRGGLGSLFYLTLDVLETDCHVLRKKAWQDCGMRIFFESVYGQCKAIFYMNNPSRVLYLAAYNCTLRPVSKKKIYMTCPDCPSSIPTDSSNHQVLEAATESLAKYKNENTSKQYSLFKVTRASSQWVVGPSYFVEYLIKESPCTKSQASSCSLQSSDSVPVGLCKGSLTRTHWEKFVSVTCDFFESQAPATGSENSAVNQKPTNLPKVEESQQKNTPPTDSPSKAGPRGSVQYLPDLDDKNSQEKGPQEAFPVHLDLTTNPQGETLDISFLFLEPMEEKLVVLPFPKEKARTAECPGPAQNASPLVLPP

• Full Name: fetuin B
• Calculated Molecular Weight: 382 aa, 42 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC074734
• Gene Symbol: Fetuin B
• Gene ID (NCBI): 26998
• RRID: AB_2882319
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UGM5
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Fetuins A and B are both members of the cystatin superfamily. They are mainly expressed in the liver (hepatokines), as well as other tissues such as the kidneys, tongue, and placenta. Fetuin-B has been identified as a novel adipokine/hepatokine that significantly increases hepatic steatosis and mediates impaired insulin action and glucose intolerance. (PMID: 36915725, PMID: 35702137)