hCG Beta Polyclonal antibody, PBS Only

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• hCG Beta Polyclonal antibody, PBS Only (33278-1-PBS)

• 

  WB analysis using 33278-1-AP (same clone as 33278-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 33278-1-AP (hCG Beta antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. Negative control: human heart tissue, human kidney tissue. This data was developed using the same antibody clone with 33278-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 33278-1-AP (same clone as 33278-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 33278-1-AP (hCG Beta antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33278-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 33278-1-AP (same clone as 33278-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 33278-1-AP (hCG Beta antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33278-1-PBS in a different storage buffer formulation.

hCG Beta Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 33278-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

CGB5, hCG, hCGB

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Product Information

33278-1-PBS targets hCG Beta in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag38044

  Product name: Recombinant human CGB5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 21-165 aa of BC051378

  Sequence: SKEPLRPRCRPINATLAVEKEGCPVCITVNTTICAGYCPTMTRVLQGVLPALPQVVCNYRDVRFESIRLPGCPRGVNPVVSYAVALSCQCALCRRSTTDCGGPKDHPLTCDDPRFQDSSSSKAPPPSLPSPSRLPGPSDTPILPQ

• Full Name: chorionic gonadotropin, beta polypeptide 5
• Calculated Molecular Weight: 165 aa, 18 kDa
• Observed Molecular Weight: 19, 21 kDa
• GenBank Accession Number: BC051378
• Gene Symbol: CGB5
• Gene ID (NCBI): 93659
• RRID: AB_3742923
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: P0DN86
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Beta-hCG (beta-human chorionic gonadotropin) is a glycoprotein hormone produced by the placenta during pregnancy. It is a critical marker used in both obstetrics and oncology for various diagnostic and monitoring purposes. hCG is composed of two subunits: an alpha subunit (similar to other glycoprotein hormones like LH, FSH, and TSH) and a unique beta subunit (β-hCG). The beta subunit is the primary focus for diagnostic tests due to its specificity. β-hCG is initially secreted by the syncytiotrophoblast cells surrounding the developing embryo and later by the placenta.