Tested Applications
| Positive WB detected in | SGC-7901 cells, U-251 cells, U-87 MG cells |
| Positive IF-P detected in | rat brain tissue, human kidney tissue |
| Positive IF/ICC detected in | U-251 cells |
| Positive FC (Intra) detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29285-1-AP targets Nestin in WB, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag29865 Product name: Recombinant human nestin protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1450-1621 aa of NM_006617 Sequence: SSSQRGEFLESDSVSVSVPWDDSLRGAVAGAPKTALETESQDSAEPSGSEEESDPVSLEREDKVPGPLEIPSGMEDAGPGADIIGVNGQGPNLEGKSQHVNGGVMNGLEQSEEVGQGMPLVSEGDRGSPFQEEEGSALKTSWAGAPVHLGQGQFLKFTQREGDRESWSSGED Predict reactive species |
| Full Name | nestin |
| Calculated Molecular Weight | 177 kDa |
| Observed Molecular Weight | 240-280 kDa |
| GenBank Accession Number | NM_006617 |
| Gene Symbol | Nestin |
| Gene ID (NCBI) | 10763 |
| RRID | AB_2918268 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P48681 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors. Nestin can be detected as 220-240 kDa and 240-280 kDa. Studies point toward nestin heavy posttranslational modifications as possibly serving particular functions in podocytes and in a few other particular cell types(PMID: 17210924).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Nestin antibody 29285-1-AP | Download protocol |
| IF protocol for Nestin antibody 29285-1-AP | Download protocol |
| WB protocol for Nestin antibody 29285-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Nestin Polyclonal antibody (Cat# 29285-1-AP) has 6 citations published in Bioactive Materials, Journal of Translational Medicine, Brain, Behavior, and Immunity, European Journal of Immunology, Molecular and Clinical Oncology, and Cells. Research fields span neural stem cell biology, spinal cord injury repair, ischemic stroke recovery, cognitive impairment, microglial immune profiling, glioblastoma differentiation therapy, and pluripotent stem cell culture.
| Species | Application | Title |
|---|---|---|
Bioact Mater Construction of functional neural network tissue combining CBD-NT3-modified linear-ordered collagen scaffold and TrkC-modified iPSC-derived neural stem cells for spinal cord injury repair | ||
J Transl Med Nrf2 overexpression reprograms neural stem cell fate: promoting neuronal differentiation and functional recovery post-ischemic stroke via suppression of the ROS/NF-κB axis. | ||
Brain Behav Immun Microbial reconstitution reverses prenatal stress-induced cognitive impairment and synaptic deficits in rat offspring | ||
Eur J Immunol High-dimensional Spatial Immune Profiling Highlights Microglia-Like Cells in Human Dorsal Root Ganglia. | ||
Mol Clin Oncol Differentiation therapy targeting indoleamine 2,3-dioxygenase 1 in glioblastoma cells in vitro. | ||
Cells Machine Learning-Driven Automated Culture Reveals Similar Performance of mTeSR+ and StemFlex in Human iP11N Pluripotent Stem Cells. |
Reviews
What customers say
The antibody performed well in immunofluorescence on neural progenitor cells at a 1:500 dilution, earning a 5-star rating. The reviewer reported good results, indicating reliable staining and specificity for this application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Baptiste (Verified Customer) (06-08-2026) | Good results on neural progenitor cells.
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