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CoraLite®594-conjugated p120 Catenin Monoclonal antibody
p120 Catenin Monoclonal Antibody for IF
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat
Applications
IF
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
2F7H8
Cat no : CL594-66208
Synonyms
Validation Data Gallery
Tested Applications
Positive IF detected in | MCF-7 cells, human breast cancer tissue |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Product Information
CL594-66208 targets p120 Catenin in IF applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | p120 Catenin fusion protein Ag2824 |
Full Name | catenin (cadherin-associated protein), delta 1 |
Calculated molecular weight | 948 aa, 105 kDa |
Observed molecular weight | 90-120 kDa |
GenBank accession number | BC010501 |
Gene symbol | CTNND1 |
Gene ID (NCBI) | 1500 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Catenins were discovered as proteins that are linked to the cytoplasmic domain of transmembrane cadherins (PMID: 9653641). p120 catenin, also called p120 ctn or catenin delta-1, regulates cell-cell adhesion through its interaction with the cytoplasmic tail of classical and type II cadherins. p120 catenin is a tyrosine kinase substrate implicated in cell transformation by SRC, as well as in ligand-induced receptor signaling through the EGF receptor, the PDGF receptor, and the CSF1 receptor. Different expression patterns of p120 catenin in lobular and ductal carcinomas of breast have been reported: membrane stain for ductal carcinoma and cytoplasmic stain for lobular carcinoma (PMID: 24966968). Different isoforms of p120 catenin are variably expressed in different tissues as a result of alternative splicing and the use of multiple translation initiation codons (PMID: 19150613).
Protocols
Product Specific Protocols | |
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IF protocol for CL594 p120 Catenin antibody CL594-66208 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |