ChromoTek p53 C-term-Trap Agarose, kit

p53-C-term-Trap Agarose is an affinity resin for IP of p53. It consists of a p53 Nanobody/ VHH coupled to agarose beads and that binds to the C-terminus of p53.

Specificity

p53

Applications

IP, CoIP

Conjugate

Agarose

Cat no : pta2k

Synonyms

p53 C-term-Trap, p53 Trap, p53-Trap,Antigen NY CO 13, Cellular tumor antigen p53, FLJ92943, LFS1, P53, Phosphoprotein p53, TP53, TRP53, tumor protein p53, Tumor suppressor p53



Product Information

p53-C-term-Trap Agarose is an affinity resin for IP of p53. It consists of a p53 Nanobody/ VHH coupled to agarose beads and that binds to the C-terminus of p53.

ApplicationsIP, CoIP
Clonality Monoclonal
HostAlpaca
Specificity/Targetp53
Species-Reactivity: Tested on human, mouse
Epitope: 302-393 aa
Coupled VHHRecombinant, monoclonal anti-p53 C-term single domain antibody (sdAb) fragment
ConjugateAgarose
Beads~ 90 µm (cross-linked 4 % agarose beads)
Elution bufferSDS sample buffer
0.2 M glycine pH 2.5
Storage Buffer20% EtOH
Storage ConditionUpon receipt store at +4°C. Do not freeze!

Kit components

ComponentDescription
p53 C-term-Trap Agarose 20 reactions (500 µL)
Lysis buffer Optimized for cytoplasmatic proteins and mammalian cell lysis
RIPA buffer Optimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer Removal of unwanted proteins, peptides, etc.
Dilution buffer Dilution of cell lysate
Elution buffer For acidic elution

Publications

ApplicationTitle
IP

Am J Respir Crit Care Med

Long Noncoding RNA TYKRIL Plays a Role in Pulmonary Hypertension via the p53-mediated Regulation of PDGFRβ.

Authors - Christoph M Zehendner