ChromoTek p53 C-term-Trap Agarose, kit

p53-C-term-Trap Agarose is an affinity resin for IP of p53. It consists of a p53 Nanobody/ VHH coupled to agarose beads and that binds to the C-terminus of p53.

Specificity

p53

Reactivity

Human, Mouse

Applications

IP, CoIP

Conjugate

Agarose

Type

Nanobody

Cat no : pta2k

Synonyms

p53 C-term-Trap, p53 Trap, p53-Trap,Antigen NY CO 13, Cellular tumor antigen p53, FLJ92943, LFS1, P53, Phosphoprotein p53, TP53, TRP53, tumor protein p53, Tumor suppressor p53



Product Information

p53-C-term-Trap Agarose is an affinity resin for IP of p53. It consists of a p53 Nanobody/ VHH coupled to agarose beads and that binds to the C-terminus of p53.

DescriptionThe p53-C-term-Trap Agarose Kit contains p53-C-term-Trap Agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of p53.

• Immunoprecipitation of human and mice p53 isoforms alpha with p53-C-term-Trap

• Pull-down of the human p53 proteome with complementary p53-N-term- and p53-C-term-Traps, e.g. p53 isoforms and variants

• No heavy & light antibody chains in your downstream application

• Short incubation time (60 min at +4°C)

• Strong binding even under harsh washing conditions

ApplicationsIP, CoIP
Specificity/Targetp53 (isoform alpha); epitope 302-393 aa
Tested ReactivityHuman, Mouse
ConjugateAgarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
Elution bufferSDS sample buffer
0.2 M glycine pH 2.5
Type Nanobody
ClassRecombinant
HostAlpaca
Compatibility with mass spectrometryThe p53-C-term-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
RRIDAB_2631379
Storage Buffer20% ethanol
Storage ConditionUpon receipt store at +4°C. Do not freeze!

Kit components

ComponentDescription
p53 C-term-Trap Agarose20 reactions (500 µL)
Lysis bufferOptimized for cytoplasmatic proteins and mammalian cell lysis
RIPA bufferOptimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer Removal of unwanted proteins, peptides, etc.
Dilution bufferDilution of cell lysate
Elution bufferFor acidic elution

Publications

ApplicationTitle
IP

Am J Respir Crit Care Med

Long Noncoding RNA TYKRIL Plays a Role in Pulmonary Hypertension via the p53-mediated Regulation of PDGFRβ.

Authors - Christoph M Zehendner