ChromoTek p53 C-term-Trap Agarose

p53-C-term-Trap Agarose is an affinity resin for IP of p53. It consists of a p53 Nanobody/ VHH coupled to agarose beads and that binds to the C-terminus of p53.

Specificity

p53

Applications

IP, CoIP

Conjugate

Agarose

Publications

3

Cat no : pta2

Synonyms

p53 C-term-Trap, p53 Trap, p53-Trap,Antigen NY CO 13, Cellular tumor antigen p53, FLJ92943, LFS1, P53, Phosphoprotein p53, TP53, TRP53, tumor protein p53, Tumor suppressor p53

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Validation Data Gallery

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  p53-C-term-Trap Agarose for Immunoprecipitation of p53.I: Input, FT: Flow-through, B: Bound.

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Product Information

p53-C-term-Trap Agarose is an affinity resin for IP of p53. It consists of a p53 Nanobody/ VHH coupled to agarose beads and that binds to the C-terminus of p53.

• Applications: IP, CoIP
• Clonality: Monoclonal
• Host: Alpaca
• Specificity/Target: p53; Species-Reactivity: Tested on human, mouse
• Coupled VHH: Recombinant, monoclonal anti-p53 C-term single domain antibody (sdAb) fragment
• Conjugate: Agarose
• Beads: ~ 90 µm (cross-linked 4 % agarose beads)
• Elution buffer: SDS sample buffer; 0.2 M glycine pH 2.5
• Storage Buffer: 20% EtOH
• Storage Condition: Upon receipt store at +4°C. Do not freeze!

Other Formats

p53 C term-Trap Agarose kit

Publications

Application	Title
CoIP	J Virol; The ORF45 protein of Kaposi Sarcoma-associated Herpesvirus (KSHV) is an inhibitor of p53 signaling during viral reactivation.; Authors - Dina Alzhanova
IP	PLoS Pathog; Novel modulators of p53-signaling encoded by unknown genes of emerging viruses.; Authors - Dina Alzhanova
IP	Cell Death Differ; The ubiquitin ligase LIN41/TRIM71 targets p53 to antagonize cell death and differentiation pathways during stem cell differentiation.; Authors - Duong Thi Thuy Nguyen