persephin-Specific Polyclonal antibody, PBS Only

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• persephin-Specific Polyclonal antibody, PBS Only (19713-1-PBS)

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  WB analysis using 19713-1-AP (same clone as 19713-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19713-1-AP (persephin-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19713-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 19713-1-AP (same clone as 19713-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19713-1-AP (persephin-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19713-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 19713-1-AP (same clone as 19713-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 19713-1-AP (persephin-Specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19713-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 19713-1-AP (same clone as 19713-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19713-1-AP (persephin-Specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19713-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 19713-1-AP (same clone as 19713-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 19713-1-AP (persephin-Specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 19713-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 19713-1-AP (same clone as 19713-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 19713-1-AP (persephin-Specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 19713-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 19713-1-AP (same clone as 19713-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19713-1-AP (persephin-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19713-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 19713-1-AP (same clone as 19713-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19713-1-AP (persephin-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19713-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 19713-1-AP (same clone as 19713-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using persephin-Specific antibody (19713-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 19713-1-PBS in a different storage buffer formulation.

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  FC experiment of SH-SY5Y using 19713-1-AP (same clone as 19713-1-PBS)

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug persephin-Specific Polyclonal antibody (19713-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00017). This data was developed using the same antibody clone with 19713-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 19713-1-AP (same clone as 19713-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug persephin-Specific Polyclonal antibody (19713-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00017). This data was developed using the same antibody clone with 19713-1-PBS in a different storage buffer formulation.

persephin-Specific Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 19713-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

persephin, PSPN, PSP

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Product Information

19713-1-PBS targets persephin-Specific in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: persephin
• Calculated Molecular Weight: 17 kDa
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: NM_004158
• Gene Symbol: Persephin/PSPN
• Gene ID (NCBI): 5623
• RRID: AB_10640431
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O60542
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PSPN, also named as PSP, belongs to the TGF-beta family and GDNF subfamily. PSPN exhibits neurotrophic activity on mesencephalic dopaminergic and motor neurons. The antibody is specific to PSPN.