RPS27A Polyclonal antibody

RPS27A Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 14946-1-AP

Synonyms

UBCEP1, UBA80, Small ribosomal subunit protein eS31, ribosomal protein S27a, HUBCEP80

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Validation Data Gallery

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  WB analysis of mouse cerebellum using 14946-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 14946-1-AP (RPS27A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14946-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14946-1-AP (RPS27A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human endometrial cancer using 14946-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 14946-1-AP (RPS27A Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human cerebellum using 14946-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 14946-1-AP (RPS27A Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human endometrial cancer using 14946-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 14946-1-AP (RPS27A Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human cerebellum using 14946-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 14946-1-AP (RPS27A Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human endometrial cancer using 14946-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 14946-1-AP (RPS27A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 14946-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RPS27A antibody (14946-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 14946-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human RPS27A (14946-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: mouse cerebellum tissue, Jurkat cells
• Positive IHC detected in: human endometrial cancer tissue, human cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IF: See 2 publications below
• FC: See 1 publications below

Product Information

14946-1-AP targets RPS27A in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPS27A fusion protein Ag6750
• Full Name: ribosomal protein S27a
• Calculated Molecular Weight: 18 kDa
• Observed Molecular Weight: 9 kDa, 18 kDa
• GenBank Accession Number: BC001392
• Gene Symbol: RPS27A
• Gene ID (NCBI): 6233
• RRID: AB_2878093
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPS27A, also named as UBA80 and UBCEP1, can be cleaved into two chains Ubiquitin and 40S ribosomal protein S27a. Ubiquitin is a highly conserved 76-amino acid protein that plays a key role in protein degradation. Ubiquitin is synthesized as precursor proteins that consist either of polyubiquitin chains that are cleaved into moieties of the UBB or UBC types, or single ubiquitin moieties fused 5-prime to unrelated carboxyl extension proteins (UBA type) such as UBA52 and UBA80 (RPS27A) [PMID:10772958]

Publications

Species	Application	Title
	FC	Front Chem; Integration of RNA-seq and ATAC-seq analyzes the effect of low dose neutron-γ radiation on gene expression of lymphocytes from oilfield logging workers; Authors - Weiguo Li
human	WB	Mediators Inflamm; UBC Mediated by SEPT6 Inhibited the Progression of Prostate Cancer.; Authors - Ruochen Zhang
human	WB	PLoS One; Ubiquitin B in cervical cancer: critical for the maintenance of cancer stem-like cell characters.; Authors - Yuan Tian
mouse	IF	Cell Rep; The proteome and transcriptome of stress granules and P bodies during human T lymphocyte activation; Authors - Nicolas Curdy
mouse	WB	J Pineal Res; Amelioration of gamma irradiation-induced salivary gland damage in mice using melatonin; Authors - Jingming Ren
human	IF	Nat Commun; Nuclear and cytoplasmic specific RNA binding proteome enrichment and its changes upon ferroptosis induction; Authors - Haofan Sun