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14-3-3 Polyclonal antibody

14-3-3 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 14503-1-AP

Synonyms

14 3 3, 14 3 3 protein T cell, 14 3 3 protein tau, 14 3 3 protein theta, 1C5, HS1, Protein HS1, YWHAQ



Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, rat liver tissue, mouse liver tissue, mouse brain tissue, mouse heart tissue
Positive IP detected inmouse lung tissue
Positive IHC detected inmouse brain tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14503-1-AP targets 14-3-3 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen 14-3-3 fusion protein Ag5959
Full Name tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide
Calculated molecular weight 28 kDa
Observed molecular weight 31 kDa
GenBank accession numberBC056867
Gene symbol YWHAQ
Gene ID (NCBI) 10971
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

14-3-3 proteins are the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis.

Protocols

Product Specific Protocols
WB protocol for 14-3-3 antibody 14503-1-APDownload protocol
IHC protocol for 14-3-3 antibody 14503-1-APDownload protocol
IF protocol for 14-3-3 antibody 14503-1-APDownload protocol
IP protocol for 14-3-3 antibody 14503-1-APDownload protocol
FC protocol for 14-3-3 antibody 14503-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Chin Med J (Engl)

Regulatory factor X5 promotes hepatocellular carcinoma progression by transactivating tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta and suppressing apoptosis.

Authors - Dong-Bo Chen
humanIP

Mol Biol Cell

Glucocorticoids and Myosin5b loss-of-function induce heightened PKA signaling in addition to membrane traffic defects.

Authors - Radia Forteza
humanWB

Amino Acids

Quantitative proteomic dissection of a native 14-3-3ε interacting protein complex associated with hepatocellular carcinoma.

Authors - Chen Bai
humanWB

J Biol Chem

14-3-3 proteins protect AMPK-phosphorylated ten-eleven translocation-2 (TET2) from PP2A-mediated dephosphorylation.

Authors - Anirban Kundu
humanWB

Elife

Ser/Thr kinase Trc controls neurite outgrowth in Drosophila by modulating microtubule-microtubule sliding.

Authors - Rosalind Norkett
  • KD Validated