ACPP Antibody

ACPP Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15840-1-AP

Synonyms

acid phosphatase, prostate, ACP 3, ACP3, ACPP, PAP, Prostatic acid phosphatase



Tested Applications

Positive WB detected inPC-3 cells, COLO 320 cells, MCF-7 cells, mouse lung tissue, mouse testis tissue
Positive IHC detected inhuman prostate cancer tissue, human prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

15840-1-AP targets ACPP in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity chicken, Rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACPP fusion protein Ag8592
Full Name acid phosphatase, prostate
Calculated molecular weight 418aa,48 kDa
Observed molecular weight 50-60 kDa
GenBank accession number BC007460
Gene symbol ACPP
Gene ID (NCBI) 55
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACPP is also named as PAP(prostatic acid phosphatase),5'-nucleotidase,thiamine monophosphatas and Belongs to the histidine acid phosphatase family.This enzyme is a homodimeric glycoprotein with a subunit molecular mass of 50 kDa and is an androgen-stimulated secretory product of the human prostate gland(PMID:8132635).It is also a non-specific phosphatase and has been implicated in the progression of prostate cancer, which inactivates LPA in human seminal plasma(PMID:15280042). This protein has three glycosylation sites(PMID:10639192;12525165).

Protocols

Product Specific Protocols
WB protocol for ACPP antibody 15840-1-APDownload protocol
IHC protocol for ACPP antibody 15840-1-APDownload protocol
IF protocol for ACPP antibody 15840-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
RatIHC, IF

Biochem Biophys Res Commun

Dietary cholesterol affects expression of prostatic acid phosphatase in reproductive organs of male rats.

Authors - Whasun Lim
chickenIHC

Biol Reprod

Avian Prostatic Acid Phosphatase: Estrogen Regulation in the Oviduct and Epithelial Cell-Derived Ovarian Carcinomas.

Authors - Hyocheol Bae