|Positive WB detected in||PC-3 cells, COLO 320 cells, MCF-7 cells, mouse lung tissue, mouse testis tissue|
|Positive IHC detected in||human prostate cancer tissue, human prostate hyperplasia tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||PC-3 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
15840-1-AP targets ACPP in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||chicken, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ACPP fusion protein Ag8592|
|Full Name||acid phosphatase, prostate|
|Calculated molecular weight||418 aa, 48 kDa|
|Observed molecular weight||50-60 kDa|
|GenBank accession number||BC007460|
|Gene ID (NCBI)||55|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ACPP is also named as PAP(prostatic acid phosphatase),5'-nucleotidase,thiamine monophosphatas and Belongs to the histidine acid phosphatase family.This enzyme is a homodimeric glycoprotein with a subunit molecular mass of 50 kDa and is an androgen-stimulated secretory product of the human prostate gland(PMID:8132635).It is also a non-specific phosphatase and has been implicated in the progression of prostate cancer, which inactivates LPA in human seminal plasma(PMID:15280042). This protein has three glycosylation sites(PMID:10639192;12525165).
Biochem Biophys Res Commun
Dietary cholesterol affects expression of prostatic acid phosphatase in reproductive organs of male rats.
Avian Prostatic Acid Phosphatase: Estrogen Regulation in the Oviduct and Epithelial Cell-Derived Ovarian Carcinomas.