ADRBK1 Monoclonal antibody

ADRBK1 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (2)

Applications

WB, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

2F7A5

Cat no : 67067-1-Ig

Synonyms

ADRBK1, BARK, BARK1, Beta ARK 1, BETA ARK1, FLJ16718, GRK2



Tested Applications

Positive WB detected inTHP-1 cells, U-937 cells, HL-60 cells, K-562 cells, Jurkat cells
Positive IHC detected inhuman lymphoma tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inJurkat cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67067-1-Ig targets ADRBK1 in WB, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ADRBK1 fusion protein Ag5071
Full Name adrenergic, beta, receptor kinase 1
Calculated Molecular Weight 689 aa, 80 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC037963
Gene Symbol ADRBK1
Gene ID (NCBI) 156
RRIDAB_2882377
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ADRBK1 antibody 67067-1-IgDownload protocol
IHC protocol for ADRBK1 antibody 67067-1-IgDownload protocol
IF protocol for ADRBK1 antibody 67067-1-IgDownload protocol
FC protocol for ADRBK1 antibody 67067-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IF,CoIP

Phytother Res

Paeoniflorin-6'-O-benzene sulfonate protected the intestinal epithelial barrier by restoring the inhibitory effect of GRK2 and β-arrestin 2 on ERK1/2-NF-κB

Authors - Mengya Jiang