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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated BCL2 Monoclonal antibody

BCL2 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4H8C6

Cat no : CL488-60178

Synonyms

Apoptosis regulator Bcl 2, B cell CLL/lymphoma 2, BCL2, Oncogene B cell leukemia 2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CoraLite® Plus 488-conjugated BCL2 Monoclonal anti (CL488-60178)

    IF Staining of human tonsillitis using CL488-60178

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488-conjugated BCL2 antibody (CL488-60178, Clone: 4H8C6 ) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CoraLite® Plus 488-conjugated BCL2 Monoclonal anti (CL488-60178)

    IF Staining of human tonsillitis using CL488-60178

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488-conjugated BCL2 antibody (CL488-60178, Clone: 4H8C6 ) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of human appendicitis tissue using CoraLite® Plus 488-conjugated BCL2 Monoclonal anti (CL488-60178)

    IF Staining of human appendicitis using CL488-60178

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CoraLite® Plus 488 BCL2 antibody (CL488-60178, Clone: 4H8C6 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of human appendicitis tissue using CoraLite® Plus 488-conjugated BCL2 Monoclonal anti (CL488-60178)

    IF Staining of human appendicitis using CL488-60178

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CoraLite® Plus 488 BCL2 antibody (CL488-60178, Clone: 4H8C6 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CoraLite® Plus 488-conjugated BCL2 Monoclonal anti (CL488-60178)

    IF Staining of MCF-7 using CL488-60178

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 BCL2 antibody (CL488-60178, Clone: 4H8C6 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of Jurkat cells using CoraLite® Plus 488-conjugated BCL2 Monoclonal anti (CL488-60178)

    FC experiment of Jurkat using CL488-60178

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human BCL2 (CL488-60178, Clone:4H8C6) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inhuman tonsillitis tissue, MCF-7 cells, human appendicitis tissue
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-60178 targets BCL2 in IF, FC (Intra) applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen BCL2 fusion protein Ag3508
Full Name B-cell CLL/lymphoma 2
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC027258
Gene Symbol BCL2
Gene ID (NCBI) 596
RRIDAB_2883111
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BCL2 belongs to the Bcl-2 family. It suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. BCL2 regulates cell death by controlling the mitochondrial membrane permeability. It appears to function in a feedback loop system with caspases. BCL2 inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 BCL2 antibody CL488-60178Download protocol
FC protocol for CL Plus 488 BCL2 antibody CL488-60178Download protocol
Standard Protocols
Click here to view our Standard Protocols