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Cyclin H Monoclonal antibody

Cyclin H Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2C5G11

Cat no : 67065-1-Ig

Synonyms

CAK, CCNH, cyclin H, MO15 associated protein, p34, p37

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cyclin H Monoclonal antibody (67065-1-Ig)

    WB analysis using 67065-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67065-1-Ig (Cyclin H antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Cyclin H Monoclonal antibody (67065-1-Ig)

    WB analysis using 67065-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67065-1-Ig (Cyclin H antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cyclin H Monoclonal antibody (67065-1-Ig)

    IHC staining of human breast cancer using 67065-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67065-1-Ig (Cyclin H antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cyclin H Monoclonal antibody (67065-1-Ig)

    IHC staining of human breast cancer using 67065-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67065-1-Ig (Cyclin H antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using Cyclin H Monoclonal antibody (67065-1-Ig)

    IF Staining of PC-3 using 67065-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using Cyclin H antibody (67065-1-Ig, Clone: 2C5G11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of PC-3 cells using Cyclin H Monoclonal antibody (67065-1-Ig)

    FC experiment of PC-3 using 67065-1-Ig

    1X10^6 PC-3 cells were intracellularly stained with 0.4 ug Anti-Human Cyclin H (67065-1-Ig, Clone:2C5G11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, MCF-7 cells, NCCIT cells, HT-1080 cells, LNCaP cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells
Positive FC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

67065-1-Ig targets Cyclin H in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivitymouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Cyclin H fusion protein Ag28556
Full Name cyclin H
Calculated Molecular Weight 38 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC005280
Gene Symbol CCNH
Gene ID (NCBI) 902
RRIDAB_2882375
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Cyclin H antibody 67065-1-IgDownload protocol
IHC protocol for Cyclin H antibody 67065-1-IgDownload protocol
IF protocol for Cyclin H antibody 67065-1-IgDownload protocol
FC protocol for Cyclin H antibody 67065-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Genes Dev

TRF1 uses a noncanonical function of TFIIH to promote telomere replication

Authors - Zhe Yang
View Article