• Featured Product
  • KD/KO Validated

ENO1 Polyclonal antibody

ENO1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 11204-1-AP

Synonyms

Alpha enolase, C myc promoter binding protein, ENO1, ENO1L1, Enolase 1, enolase 1, (alpha), MBP 1, MBPB1, MPB 1, MPB1, NNE, Non neural enolase, Phosphopyruvate hydratase, Plasminogen binding protein, PPH



Tested Applications

Positive WB detected inhuman skeletal muscle tissue, PC-3 cells, SGC-7901 cells, mouse skeletal muscle tissue
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman colon cancer tissue, human liver cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11204-1-AP targets ENO1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ENO1 fusion protein Ag1692
Full Name enolase 1, (alpha)
Calculated molecular weight 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC015641
Gene symbol ENO1
Gene ID (NCBI) 2023
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Enolase is an important glycolytic enzyme involved in the interconversion of 2-phosphoglycerate to phosphoenolpyruvate.Enolase were down-regulated in oxLDL-treated cells or in VLDL-treated cells .And it identified which are associated with glucose metabolism were down-regulated in the process of foam cells formation.(19756395)

Protocols

Product Specific Protocols
WB protocol for ENO1 antibody 11204-1-APDownload protocol
IHC protocol for ENO1 antibody 11204-1-APDownload protocol
IF protocol for ENO1 antibody 11204-1-APDownload protocol
IP protocol for ENO1 antibody 11204-1-APDownload protocol
FC protocol for ENO1 antibody 11204-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Purification of cross-linked RNA-protein complexes by phenol-toluol extraction.

Authors - Erika C Urdaneta
WB

Biomolecules

PMID: 31480692

Authors - Xixi Guo
humanIHC

J Mol Histol

Gamma-enolase predicts lung damage in severe acute pancreatitis-induced acute lung injury.

Authors - Lawrence Owusu
humanWB

Nat Metab

Myc-mediated SDHA acetylation triggers epigenetic regulation of gene expression and tumorigenesis.

Authors - Shi-Ting Li
ratWB

Can J Physiol Pharmacol

Upregulation of α-enolase protects cardiomyocytes from phenylephrine-induced hypertrophy.

Authors - Si Gao
  • KD Validated
humanWB

Mol Cells

Proteomic analysis of the triglyceride-rich lipoprotein-laden foam cells.

Authors - Lu Yanjun Y