Tested Applications
| Positive WB detected in | A549 cells, HEK-293 cells, HeLa cells, human spleen tissue, MCF-7 cells, Calu-1 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, MDCK cells, HEK-293 cells, HeLa cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11308-1-AP targets GOLGA2/GM130 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, canine samples.
| Tested Reactivity | human, canine |
| Cited Reactivity | human, pig, monkey, zebrafish, hamster, sheep |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1848 Product name: Recombinant human GM130;GOLGA2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 361-678 aa of BC014188 Sequence: LRAQLSLMAHPGEGDGLDREEEEDEEEEEEEAVAVPQPMPSIPEDLESREAMVAFFNSAVASAEEEQARLRGQLKEQRVRCRRLAHLLASAQKEPEAAAPAPGTGGDSVCGETHRALQGAMEKLQSRFMELMQEKADLKERVEELEHRCIQLSGETDTIGEYIALYQSQRAVLKERHREKEEYISRLAQDKEEMKVKLLELQELVLRLVGDRNEWHGRFLAAAQNPADEPTSGAPAPQELGAANQQGDLCEVSLAGSVEPAQGEAREGSPRDNPTAQQIMQLLREMQNPRERPGLGSNPCIPFFYRADENDEVKITVI Predict reactive species |
| Full Name | golgi autoantigen, golgin subfamily a, 2 |
| Calculated Molecular Weight | 111 kDa |
| Observed Molecular Weight | 130 kDa |
| GenBank Accession Number | BC014188 |
| Gene Symbol | GM130 |
| Gene ID (NCBI) | 2801 |
| RRID | AB_2115327 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q08379 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GOLGA2, also known as GM130, is a 130 kDa cis-Golgi matrix protein which is one component of the detergent and salt resistant Golgi matrix. It is a peripheral membrane protein highly bound to Golgi membrane and localized mainly at the cytoplasmic face of cis-Golgi membrane. Together with its interacting partner proteins, including p115, giantin, GRASP65, and Rab GTPase, GOLGA2/GM130 is involved in the regulation of ER-to-Golgi transport and also in the maintenance of the Golgi structure. Emerging evidence suggest that the GOLGA2/GM130 has potential roles in the control of glycosylation, cell cycle progression, and higher order cell functions such as cell polarization and directed cell migration. (PMID: 20197635)
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for GOLGA2/GM130 antibody 11308-1-AP | Download protocol |
| IF protocol for GOLGA2/GM130 antibody 11308-1-AP | Download protocol |
| IHC protocol for GOLGA2/GM130 antibody 11308-1-AP | Download protocol |
| IP protocol for GOLGA2/GM130 antibody 11308-1-AP | Download protocol |
| WB protocol for GOLGA2/GM130 antibody 11308-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GOLGA2/GM130 polyclonal antibody (Cat# 11308-1-AP) has 241 citations published in prestigious journals including Nature, Cell, Nature Cell Biology, Nature Communications, Science Advances, Molecular Cell, EMBO Journal, Journal of Clinical Investigation, Immunity, and Cell Metabolism. Research fields covered include Golgi/ER membrane trafficking, lipid homeostasis, autophagy, extracellular vesicle/exosome biology, cancer biology, innate immunity, ferroptosis, neurodegeneration, viral pathogenesis, and tissue regeneration. Primary applications are WB, IF, IP, and IHC.
| Species | Application | Title |
|---|---|---|
Cell Unraveling cellular complexity with transient adapters in highly multiplexed super-resolution imaging | ||
Reviews
What customers say
All three reviewers gave 5-star ratings. The antibody performed well in both Western blot and immunofluorescence applications. One user reported a very strong and specific Golgi signal in SH-SY5Y cells at 1:1000 dilution with 1-hour RT incubation. Another confirmed excellent WB performance. A third user successfully used it at 1:300 for co-staining human RPE cells with EEA1 and DAPI after PFA/Permeabilization fixation. Overall, users found the antibody reliable, specific, and easy to use across multiple applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Tatyana (Verified Customer) (06-15-2025) | Incubation at RT for 1 h in 5% goat serum/PBST. Very strong specific signal of Golgi apparatus.
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Boyan (Verified Customer) (04-02-2023) | very good for WB.
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Stephen (Verified Customer) (10-01-2019) | RPE cells fixed with 4% PFA Perm. by 0.3% tx100 for 5 min blocked with 1% BSA in 1XPBS for 2 hours GM130 antibody (green) incubated 1:300 and EEA1 antibody (purple) overnight at 4 degrees in 1%BSA in 1x PBS. Co-stained with DAPI (blue) visualize DNA
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