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  • KD/KO Validated

GOLGA2/GM130 Polyclonal antibody

GOLGA2/GM130 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, Canine and More (2)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 11308-1-AP

Synonyms

Cell and organelle markers, GM130, GM130 autoantigen, GOLGA2, Golgi Marker, Golgin 95, Golgin subfamily A member 2



Tested Applications

Positive WB detected inA549 cells, HeLa cells, human spleen tissue, HEK-293 cells, MCF-7 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HEK-293 cells, HepG2 cells, MDCK cells
Positive FC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11308-1-AP targets GOLGA2/GM130 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, Canine samples.

Tested Reactivity human, Canine
Cited Reactivityhuman, monkey, Hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GOLGA2/GM130 fusion protein Ag1848
Full Name golgi autoantigen, golgin subfamily a, 2
Calculated Molecular Weight 111 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC014188
Gene Symbol GOLGA2
Gene ID (NCBI) 2801
RRIDAB_2115327
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GOLGA2, also known as GM130, is a 130 kDa cis-Golgi matrix protein which is one component of the detergent and salt resistant Golgi matrix. It is a peripheral membrane protein highly bound to Golgi membrane and localized mainly at the cytoplasmic face of cis-Golgi membrane. Together with its interacting partner proteins, including p115, giantin, GRASP65, and Rab GTPase, GOLGA2/GM130 is involved in the regulation of ER-to-Golgi transport and also in the maintenance of the Golgi structure. Emerging evidence suggest that the GOLGA2/GM130 has potential roles in the control of glycosylation, cell cycle progression, and higher order cell functions such as cell polarization and directed cell migration. (PMID: 20197635)

Protocols

Product Specific Protocols
WB protocol for GOLGA2/GM130 antibody 11308-1-APDownload protocol
IHC protocol for GOLGA2/GM130 antibody 11308-1-APDownload protocol
IF protocol for GOLGA2/GM130 antibody 11308-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nature

ER proteins decipher the tubulin code to regulate organelle distribution

Authors - Pengli Zheng

Immunity

The nucleotide receptor STING translocates to the phagosomes to negatively regulate anti-fungal immunity

Authors - Tian Chen
humanIF

Nat Methods

Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM).

Authors - Marine H Laporte
WB

Cell Metab

Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans.

Authors - Xiao Wang
humanWB,IF

Cell Metab

TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis.

Authors - Dong Huang
IF

Nat Cell Biol

Manganese regulation of COPII condensation controls circulating lipid homeostasis

Authors - Xiao Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Boyan (Verified Customer) (04-02-2023)

very good for WB.

  • Applications: Western Blot
FH

Stephen (Verified Customer) (10-01-2019)

RPE cells fixed with 4% PFA Perm. by 0.3% tx100 for 5 min blocked with 1% BSA in 1XPBS for 2 hours GM130 antibody (green) incubated 1:300 and EEA1 antibody (purple) overnight at 4 degrees in 1%BSA in 1x PBS. Co-stained with DAPI (blue) visualize DNA

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:300
  • Cell Tissue Type: RPE (human)