CoraLite® Plus 647-conjugated GOLGA2/GM130 Polyclonal antibody

GOLGA2/GM130 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, canine

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-11308

Synonyms

GM130, GOLGA2, Golgin 95, GM130;GOLGA2, GM130 autoantigen

---

Validation Data Gallery

• 

  IF Staining of HepG2 using CL647-11308

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 647 GOLGA2/GM130 antibody (CL647-11308) at dilution of 1:200.

• 

  FC experiment of HeLa using CL647-11308

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human GOLGA2/GM130 (CL647-11308) (red), or 0.2 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Tested Applications

• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-11308 targets GOLGA2/GM130 in IF/ICC, FC (Intra) applications and shows reactivity with human, canine samples.

• Tested Reactivity: human, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GOLGA2/GM130 fusion protein Ag1848
• Full Name: golgi autoantigen, golgin subfamily a, 2
• Calculated Molecular Weight: 111 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC014188
• Gene Symbol: GM130
• Gene ID (NCBI): 2801
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GOLGA2, also known as GM130, is a 130 kDa cis-Golgi matrix protein, one component of the detergent and salt-resistant Golgi matrix. It is a peripheral membrane protein highly bound to the Golgi membrane and localized mainly at the cytoplasmic face of the cis-Golgi membrane. Together with its interacting partner proteins, including p115, giantin, GRASP65, and Rab GTPase, GOLGA2/GM130 is involved in regulating ER-to-Golgi transport and maintaining the Golgi structure. Emerging evidence suggests that the GOLGA2/GM130 has potential roles in the control of glycosylation, cell cycle progression, and higher-order cell functions such as cell polarization and directed cell migration. (PMID: 20197635)