Tested Applications
| Positive IF/ICC detected in | HepG2 cells, HeLa cells |
| Positive FC (Intra) detected in | HeLa cells, HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL647-11308 targets GOLGA2/GM130 in IF/ICC, FC (Intra) applications and shows reactivity with human, canine samples.
| Tested Reactivity | human, canine |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1848 Product name: Recombinant human GM130;GOLGA2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 361-678 aa of BC014188 Sequence: LRAQLSLMAHPGEGDGLDREEEEDEEEEEEEAVAVPQPMPSIPEDLESREAMVAFFNSAVASAEEEQARLRGQLKEQRVRCRRLAHLLASAQKEPEAAAPAPGTGGDSVCGETHRALQGAMEKLQSRFMELMQEKADLKERVEELEHRCIQLSGETDTIGEYIALYQSQRAVLKERHREKEEYISRLAQDKEEMKVKLLELQELVLRLVGDRNEWHGRFLAAAQNPADEPTSGAPAPQELGAANQQGDLCEVSLAGSVEPAQGEAREGSPRDNPTAQQIMQLLREMQNPRERPGLGSNPCIPFFYRADENDEVKITVI Predict reactive species |
| Full Name | golgi autoantigen, golgin subfamily a, 2 |
| Calculated Molecular Weight | 111 kDa |
| Observed Molecular Weight | 130 kDa |
| GenBank Accession Number | BC014188 |
| Gene Symbol | GM130 |
| Gene ID (NCBI) | 2801 |
| RRID | AB_2934854 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
| Excitation Laser | Red Laser (633 nm) |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q08379 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GOLGA2, also known as GM130, is a 130 kDa cis-Golgi matrix protein, one component of the detergent and salt-resistant Golgi matrix. It is a peripheral membrane protein highly bound to the Golgi membrane and localized mainly at the cytoplasmic face of the cis-Golgi membrane. Together with its interacting partner proteins, including p115, giantin, GRASP65, and Rab GTPase, GOLGA2/GM130 is involved in regulating ER-to-Golgi transport and maintaining the Golgi structure. Emerging evidence suggests that the GOLGA2/GM130 has potential roles in the control of glycosylation, cell cycle progression, and higher-order cell functions such as cell polarization and directed cell migration. (PMID: 20197635)
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 647 GOLGA2/GM130 antibody CL647-11308 | Download protocol |
| IF protocol for CL Plus 647 GOLGA2/GM130 antibody CL647-11308 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GOLGA2/GM130 antibody (CL647-11308) has 2 citations. They appear in Advanced Science (2026) and Cell Reports (2025). Research fields include immunology/vaccinology—LNP-mRNA gE antigen against varicella zoster virus—and cancer/neuroscience—SRRM3-regulated alternative splicing in pancreatic neuroendocrine tumors. Both used the antibody for immunofluorescence in human samples.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Potent and Long-Lasting Immunogenicity Generated by LNP-mRNA gE Antigen Against Varicella Zoster Virus via an AI-Assisted Pipeline. | ||
Cell Rep An SRRM3-regulated neural alternative splicing program is subverted to promote tumor progression in pancreatic neuroendocrine cells |
Reviews
What customers say
The single review for CL647-11308 (rated 5/5) reports excellent performance in immunofluorescence on human HeLa cells at a 1:500 dilution. The reviewer notes that mouse fibroblasts work well at a lower 1:100 dilution but show some background signal. Overall, the antibody is well-received for IF applications, particularly in human cell systems.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Jacob (Verified Customer) (09-04-2025) | Really great for human cells, mouse cells require 1:100 dilution and have some background too.
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