CoraLite®555-conjugated GOLGA2/GM130 Polyclonal antibody
GOLGA2/GM130 Polyclonal Antibody for FC (Intra), IF
Host / Isotype
Rabbit / IgG
Reactivity
human, Canine
Applications
IF, FC (Intra)
Conjugate
CoraLite®555 Fluorescent Dye
Cat no : CL555-11308
Synonyms
Validation Data Gallery
Tested Applications
Positive IF detected in | HepG2 cells, HeLa cells |
Positive FC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Product Information
CL555-11308 targets GOLGA2/GM130 in IF, FC (Intra) applications and shows reactivity with human, Canine samples.
Tested Reactivity | human, Canine |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | GOLGA2/GM130 fusion protein Ag1848 |
Full Name | golgi autoantigen, golgin subfamily a, 2 |
Calculated molecular weight | 111 kDa |
Observed molecular weight | 130 kDa |
GenBank accession number | BC014188 |
Gene symbol | GOLGA2 |
Gene ID (NCBI) | 2801 |
Conjugate | CoraLite®555 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 557 nm / 570nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GOLGA2, also known as GM130, is a 130 kDa cis-Golgi matrix protein which is one component of the detergent and salt resistant Golgi matrix. It is a peripheral membrane protein highly bound to Golgi membrane and localized mainly at the cytoplasmic face of cis-Golgi membrane. Together with its interacting partner proteins, including p115, giantin, GRASP65, and Rab GTPase, GOLGA2/GM130 is involved in the regulation of ER-to-Golgi transport and also in the maintenance of the Golgi structure. Emerging evidence suggest that the GOLGA2/GM130 has potential roles in the control of glycosylation, cell cycle progression, and higher order cell functions such as cell polarization and directed cell migration. (PMID: 20197635)
Protocols
Product Specific Protocols | |
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IF protocol for CL555 GOLGA2/GM130 antibody CL555-11308 | Download protocol |
FC protocol for CL555 GOLGA2/GM130 antibody CL555-11308 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |