CoraLite®555-conjugated GOLGA2/GM130 Polyclonal antibody

GOLGA2/GM130 Polyclonal Antibody for IF, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, Canine

Applications

IF, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

Cat no : CL555-11308

Synonyms

Cell and organelle markers, GM130, GM130 autoantigen, GOLGA2, Golgi Marker, Golgin 95, Golgin subfamily A member 2



Tested Applications

Positive IF detected inHeLa cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-11308 targets GOLGA2/GM130 in IF, FC (Intra) applications and shows reactivity with human, Canine samples.

Tested Reactivity human, Canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GOLGA2/GM130 fusion protein Ag1848
Full Name golgi autoantigen, golgin subfamily a, 2
Calculated Molecular Weight 111 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC014188
Gene Symbol GOLGA2
Gene ID (NCBI) 2801
RRIDAB_2919599
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GOLGA2, also known as GM130, is a 130 kDa cis-Golgi matrix protein which is one component of the detergent and salt resistant Golgi matrix. It is a peripheral membrane protein highly bound to Golgi membrane and localized mainly at the cytoplasmic face of cis-Golgi membrane. Together with its interacting partner proteins, including p115, giantin, GRASP65, and Rab GTPase, GOLGA2/GM130 is involved in the regulation of ER-to-Golgi transport and also in the maintenance of the Golgi structure. Emerging evidence suggest that the GOLGA2/GM130 has potential roles in the control of glycosylation, cell cycle progression, and higher order cell functions such as cell polarization and directed cell migration. (PMID: 20197635)

Protocols

Product Specific Protocols
IF protocol for CL555 GOLGA2/GM130 antibody CL555-11308Download protocol
Standard Protocols
Click here to view our Standard Protocols