|Positive WB detected in||mouse liver tissue, mouse kidney tissue|
|Positive IP detected in||mouse kidney tissue|
|Positive IHC detected in||human skeletal muscle tissue, mouse brain tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
16757-1-AP targets GPT2 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GPT2 fusion protein Ag10273|
|Full Name||glutamic pyruvate transaminase (alanine aminotransferase) 2|
|Calculated molecular weight||523 aa, 58 kDa|
|Observed molecular weight||58 kDa, 47 kDa|
|GenBank accession number||BC062555|
|Gene ID (NCBI)||84706|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
GPT2 (Alanine aminotransferase 2; ALT2) is a key enzyme in intermediatary metabolism, catalysing the reversible transfer of the amino group of glutamate to pyruvate, yielding alanine and ketoglutarate. GPT2 protein localizes to mitochondria, and is mainly expressed in the pancreas, brain, adrenal gland, skeletal muscle and heart. Serum GPT2 is a useful marker for liver damage. Recently mutation of GPT2 has been reported to be a cause of neural disorder (27601654). 16757-1-AP antibody recognizes two different isoforms of GPT2 protein.
|Product Specific Protocols|
|WB protocol for GPT2 antibody 16757-1-AP||Download protocol|
|IHC protocol for GPT2 antibody 16757-1-AP||Download protocol|
|IF protocol for GPT2 antibody 16757-1-AP||Download protocol|
|IP protocol for GPT2 antibody 16757-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Mitochondrial GPT2 plays a pivotal role in metabolic adaptation to the perturbation of mitochondrial glutamine metabolism.
Am J Respir Cell Mol Biol
Glutamine Metabolism is Required for Collagen Protein Synthesis in Lung Fibroblasts.
Transcriptomic and metabolic analyses reveal salvage pathways in creatine-deficient AGAT(-/-) mice.
Ann Transl Med
Enhanced glutamine utilization mediated by SLC1A5 and GPT2 is an essential metabolic feature of colorectal signet ring cell carcinoma with therapeutic potential.
Exosome-transmitted microRNA-133b inhibited bladder cancer proliferation by upregulating dual-specificity protein phosphatase 1.
Metabolic consequences of HIF silencing in a triple negative human breast cancer xenograft.