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  • Featured Product
  • KD/KO Validated

GPT2 Polyclonal antibody

GPT2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC (Intra), FC, ELISA

Conjugate

Unconjugated

Cat no : 16757-1-AP

Synonyms

AAT2, Alanine aminotransferase 2, ALAT2, ALT2, GPT 2, GPT2

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using GPT2 Polyclonal antibody (16757-1-AP)

    WB analysis of HepG2 using 16757-1-AP

    WB result of GPT2 antibody (16757-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GPT2 transfected HepG2 cells.

  • Western Blot (WB) analysis of various lysates using GPT2 Polyclonal antibody (16757-1-AP)

    WB analysis using 16757-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16757-1-AP (GPT2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using GPT2 Polyclonal antibody (16757-1-AP)

    WB analysis of mouse kidney using 16757-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16757-1-AP (GPT2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse kidney tissue using GPT2 Polyclonal antibody (16757-1-AP)

    IP experiment of mouse kidney using 16757-1-AP

    IP result of anti-GPT2 (IP:16757-1-AP, 4ug; Detection:16757-1-AP 1:500) with mouse kidney tissue lysate 7200ug.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using GPT2 Polyclonal antibody (16757-1-AP)

    IHC staining of human skeletal muscle using 16757-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16757-1-AP (GPT2 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using GPT2 Polyclonal antibody (16757-1-AP)

    IHC staining of human skeletal muscle using 16757-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16757-1-AP (GPT2 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GPT2 Polyclonal antibody (16757-1-AP)

    IHC staining of mouse brain using 16757-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16757-1-AP (GPT2 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GPT2 Polyclonal antibody (16757-1-AP)

    IHC staining of mouse brain using 16757-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16757-1-AP (GPT2 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GPT2 Polyclonal antibody (16757-1-AP)

    IF Staining of HepG2 using 16757-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GPT2 antibody (16757-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Flow cytometry (FC) experiment of HepG2 cells using GPT2 Polyclonal antibody (16757-1-AP)

    FC experiment of HepG2 using 16757-1-AP

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human GPT2 (16757-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inmouse liver tissue, mouse kidney tissue, HepG2 cells
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman skeletal muscle tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16757-1-AP targets GPT2 in WB, IP, IHC, IF, FC (Intra), FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GPT2 fusion protein Ag10273
Full Name glutamic pyruvate transaminase (alanine aminotransferase) 2
Calculated Molecular Weight 523 aa, 58 kDa
Observed Molecular Weight 58 kDa, 47 kDa
GenBank Accession NumberBC062555
Gene Symbol GPT2
Gene ID (NCBI) 84706
RRIDAB_2112098
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPT2 (Alanine aminotransferase 2; ALT2) is a key enzyme in intermediatary metabolism, catalysing the reversible transfer of the amino group of glutamate to pyruvate, yielding alanine and ketoglutarate. GPT2 protein localizes to mitochondria, and is mainly expressed in the pancreas, brain, adrenal gland, skeletal muscle and heart. Serum GPT2 is a useful marker for liver damage. Recently mutation of GPT2 has been reported to be a cause of neural disorder (27601654). 16757-1-AP antibody recognizes two different isoforms of GPT2 protein.

Protocols

Product Specific Protocols
WB protocol for GPT2 antibody 16757-1-APDownload protocol
IHC protocol for GPT2 antibody 16757-1-APDownload protocol
IF protocol for GPT2 antibody 16757-1-APDownload protocol
IP protocol for GPT2 antibody 16757-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Cell Metab

Rewiring of Glutamine Metabolism Is a Bioenergetic Adaptation of Human Cells with Mitochondrial DNA Mutations.

Authors - Qiuying Chen
View Article
mouseWB

Cell Res

Glutaminase GLS1 senses glutamine availability in a non-enzymatic manner triggering mitochondrial fusion.

Authors - Wei-Feng Cai
  • KD Validated
View Article
humanWB

Nat Commun

Sirtuin5 contributes to colorectal carcinogenesis by enhancing glutaminolysis in a deglutarylation-dependent manner.

Authors - Yun-Qian Wang
View Article
humanWB

Cancer Res

Targeting BCAT1 combined with α-ketoglutarate triggers metabolic synthetic lethality in glioblastoma.

Authors - Bo Zhang
View Article
MOUSEWB,IF

Proc Natl Acad Sci U S A

Mutations in mitochondrial enzyme GPT2 cause metabolic dysfunction and neurological disease with developmental and progressive features.

Authors - Qing Ouyang
View Article
humanIHC

Cell Rep

Thyroid hormone regulates glutamine metabolism and anaplerotic fluxes by inducing mitochondrial glutamate aminotransferase GPT2

Authors - Annunziata Gaetana Cicatiello
View Article