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GPT2 Antibody

GPT2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16757-1-AP

Synonyms

AAT2, Alanine aminotransferase 2, ALT2, GPT 2, GPT2



Tested Applications

Positive WB detected inmouse liver tissue, mouse kidney tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman skeletal muscle tissue, mouse brain tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

16757-1-AP targets GPT2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GPT2 fusion protein Ag10273
Full Name glutamic pyruvate transaminase (alanine aminotransferase) 2
Calculated molecular weight 523aa,58 kDa
Observed molecular weight 58 kDa or 47 kDa
GenBank accession number BC062555
Gene symbol GPT2
Gene ID (NCBI) 84706
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GPT2 (Alanine aminotransferase 2; ALT2) is a key enzyme in intermediatary metabolism, catalysing the reversible transfer of the amino group of glutamate to pyruvate, yielding alanine and ketoglutarate. GPT2 protein localizes to mitochondria, and is mainly expressed in the pancreas, brain, adrenal gland, skeletal muscle and heart. Serum GPT2 is a useful marker for liver damage. Recently mutation of GPT2 has been reported to be a cause of neural disorder (27601654). 16757-1-AP antibody recognizes two different isoforms of GPT2 protein.

Protocols

Product Specific Protocols
WB protocol for GPT2 antibody 16757-1-APDownload protocol
IHC protocol for GPT2 antibody 16757-1-APDownload protocol
IF protocol for GPT2 antibody 16757-1-APDownload protocol
IP protocol for GPT2 antibody 16757-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncogene

Mitochondrial GPT2 plays a pivotal role in metabolic adaptation to the perturbation of mitochondrial glutamine metabolism.

Authors - Minjoong Kim
humanWB

Am J Respir Cell Mol Biol

Glutamine Metabolism is Required for Collagen Protein Synthesis in Lung Fibroblasts.

Authors - Robert B Hamanaka
mouseWB

Amino Acids

Transcriptomic and metabolic analyses reveal salvage pathways in creatine-deficient AGAT(-/-) mice.

Authors - Malte Stockebrand
humanWB,IHC

Ann Transl Med

Enhanced glutamine utilization mediated by SLC1A5 and GPT2 is an essential metabolic feature of colorectal signet ring cell carcinoma with therapeutic potential.

Authors - Renjie Wang
  • KD Validated
human,mouseWB

Cancer Med

Exosome-transmitted microRNA-133b inhibited bladder cancer proliferation by upregulating dual-specificity protein phosphatase 1.

Authors - Xiaoxiao Cai
humanWB

Oncotarget

Metabolic consequences of HIF silencing in a triple negative human breast cancer xenograft.

Authors - Santosh K Bharti