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GRP78/BIP Polyclonal antibody

GRP78/BIP Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 11587-1-AP

Synonyms

BIP, GRP 78, GRP78, HSP A5, HSPA5, MIF2



Tested Applications

Positive WB detected inHEK-293 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inmouse kidney tissue, human breast cancer tissue, human pancreas cancer tissue, mouse heart tissue, rat brain tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11587-1-AP targets GRP78/BIP in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity Bovine, chicken, cow, fish, Hamster, human, mouse, porcine, rat, sika deer
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GRP78/BIP fusion protein Ag2188
Full Name heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
Calculated molecular weight 78 kDa
Observed molecular weight 70-78 kDa
GenBank accession numberBC020235
Gene symbol HSPA5
Gene ID (NCBI) 3309
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER).It is a constitutively expressed resident protein of the ER in all eukaryotic cells. Recently it has been reported that GRP78 is associated with apoptosis or inhibition of cancer cell growth. 11587-1-AP antibody recognizes one or two bands with MW 70-78 kDa for the signal peptide of GRP78 protein can be finally cleaved.

Protocols

Product Specific Protocols
WB protocol for GRP78/BIP antibody 11587-1-APDownload protocol
IHC protocol for GRP78/BIP antibody 11587-1-APDownload protocol
IF protocol for GRP78/BIP antibody 11587-1-APDownload protocol
IP protocol for GRP78/BIP antibody 11587-1-APDownload protocol
FC protocol for GRP78/BIP antibody 11587-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Pharmacol Res

Pharmacological preconditioning with the cellular stress inducer thapsigargin protects against experimental sepsis.

Authors - Yaping Wei
mouseWB

Oxid Med Cell Longev

Methane-Rich Saline Ameliorates Sepsis-Induced Acute Kidney Injury through Anti-Inflammation, Antioxidative, and Antiapoptosis Effects by Regulating Endoplasmic Reticulum Stress.

Authors - Yifan Jia
sika deerWB

J Proteomics

Quantitative proteomic analysis of deer antler stem cells as a model of mammalian organ regeneration.

Authors - Zhen Dong
porcineWB

J Virol

Porcine circovirus type 2 induces ORF3-independent mitochondrial apoptosis via PERK activation and elevation of cytosolic calcium.

Authors - Yikai Zhang
humanWB

J Neurochem

Quantitative interaction proteomics reveals differences in the interactomes of amyloid precursor protein isoforms.

Authors - Robert J Andrew
ratIHC

Acta Biochim Biophys Sin (Shanghai)

Chronic intermittent hypoxia accelerates liver fibrosis in rats with combined hypoxia and nonalcoholic steatohepatitis via angiogenesis rather than endoplasmic reticulum stress.

Authors - Wei Wu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Kevin (Verified Customer) (01-24-2019)

Works pretty good in HepG2 cells. One single band was observed.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HepG2
FH

Sylvain (Verified Customer) (12-03-2018)

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:300
  • Cell Tissue Type: colon cancer cell lines
FH

Natalie (Verified Customer) (11-20-2018)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: Rat Dorsal Root Ganglia
GRP78/BIP Antibody Western Blot, validation (1:5000 dilution) in Rat Dorsal Root Ganglia (Cat no:11587-1-AP)