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KD/KO Validated

GRP78/BIP Polyclonal antibody

GRP78/BIP Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 11587-1-AP

Publications(955)

Reviews (9)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (9)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Synonyms

BIP, GRP78, HSPA5, 78 kDa glucose-regulated protein, Binding-immunoglobulin protein

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

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Proteintech Guarantee

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 11587-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11587-1-AP (GRP78/BIP antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 11587-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11587-1-AP (GRP78/BIP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 11587-1-AP
Caco-2 cells were subjected to SDS PAGE followed by western blot with 11587-1-AP (GRP78/BIP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 11587-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11587-1-AP (GRP78/BIP antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of MCF-7 using 11587-1-AP
IP result of anti-GRP78/BIP (IP:11587-1-AP, 3ug; Detection:11587-1-AP 1:1000) with MCF-7 cells lysate 3250ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human pancreas cancer using 11587-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11587-1-AP (GRP78/BIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse heart using 11587-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11587-1-AP (GRP78/BIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse heart using 11587-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11587-1-AP (GRP78/BIP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse kidney using 11587-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11587-1-AP (GRP78/BIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse kidney using 11587-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11587-1-AP (GRP78/BIP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon cancer using 11587-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 11587-1-AP (GRP78/BIP antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon cancer using 11587-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 11587-1-AP (GRP78/BIP antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human breast cancer using 11587-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11587-1-AP (GRP78/BIP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human breast cancer using 11587-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11587-1-AP (GRP78/BIP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse heart using 11587-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11587-1-AP (GRP78/BIP antibody at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse heart using 11587-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11587-1-AP (GRP78/BIP antibody at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse kidney using 11587-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11587-1-AP (GRP78/BIP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat brain using 11587-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 11587-1-AP (GRP78/BIP antibody) at dilution of 1:200 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat brain using 11587-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 11587-1-AP (GRP78/BIP antibody) at dilution of 1:200 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HepG2 using 11587-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GRP78/BIP antibody (11587-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HepG2 using 11587-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GRP78/BIP antibody (11587-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HepG2 using 11587-1-AP
Immunofluorescent analysis of (4% PFA) fixed Thapsigargin treated HepG2 cells using GRP78/BIP antibody (11587-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HepG2 using 11587-1-AP
Immunofluorescent analysis of HepG2 cells using 11587-1-AP (GRP78/BIP antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of HeLa using 11587-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human GRP78/BIP (11587-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (955)
Protocols
Reviews (9)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	BxPC-3 cells, Caco-2 cells, HEK-293 cells, HeLa cells, HepG2 cells, mouse brain tissue, mouse liver tissue, rat brain tissue, rat liver tissue, HT-29 cells
Positive IP detected in	MCF-7 cells
Positive IHC detected in	human pancreas cancer tissue, human breast cancer tissue, mouse kidney tissue, rat brain tissue, mouse heart tissue, human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells, Thapsigargin treated HepG2 cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11587-1-AP targets GRP78/BIP in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	canine, chicken, bovine, hamster, goat, fish, deer, c.elegans, carp
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2188 Product name: Recombinant human GRP78,BIP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 351-654 aa of BC020235 Sequence: LKKSDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGDQDTGDLVLLDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHLLGTFDLTGIPPAPRGVPQIEVTFEIDVNGILRVTAEDKGTGNKNKITITNDQNRLTPEEIERMVNDAEKFAEEDKKLKERIDTRNELESYAYSLKNQIGDKEKLGGKLSSEDKETMEKAVEEKIEWLESHQDADIEDFKAKKKELEEIVQPIISKLYGSAGPPPTGEEDTAEKDEL Predict reactive species
Full Name	heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
Calculated Molecular Weight	78 kDa
Observed Molecular Weight	70-78 kDa
GenBank Accession Number	BC020235
Gene Symbol	GRP78/BIP
Gene ID (NCBI)	3309
RRID	AB_2119855
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P11021
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). It is a constitutively expressed resident protein of the ER in all eukaryotic cells. Recently it has been reported that GRP78 is associated with apoptosis or inhibition of cancer cell growth. 11587-1-AP antibody recognizes one or two bands with MW 70-78 kDa for the signal peptide of GRP78 protein can be finally cleaved.

Protocols

Product Specific Protocols
FC protocol for GRP78/BIP antibody 11587-1-AP	Download protocol
IF protocol for GRP78/BIP antibody 11587-1-AP	Download protocol
IHC protocol for GRP78/BIP antibody 11587-1-AP	Download protocol
IP protocol for GRP78/BIP antibody 11587-1-AP	Download protocol
WB protocol for GRP78/BIP antibody 11587-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Species	Application	Title
human	WB	Signal Transduct Target Ther Elaiophylin triggers paraptosis and preferentially kills ovarian cancer drug-resistant cells by inducing MAPK hyperactivation Authors - Guan-Nan Li View Article
mouse,human	WB	Nature CLCC1 governs ER bilayer equilibration to maintain lipid homeostasis. Authors - Lingzhi Wu View Article
mouse,human	CoIP	Cancer Cell Tumor-resident probiotic Clostridium butyricum improves aPD-1 efficacy in colorectal cancer models by inhibiting IL-6-mediated immunosuppression. Authors - Mingxu Xie View Article
		Nature Pathology-oriented multiplexing enables integrative disease mapping Authors - Malte Kuehl View Article
	WB	Science Translation from the 5' untranslated region shapes the integrated stress response. Authors - Shelley R Starck View Article
human	WB	Science Endosomal lipid signaling reshapes the endoplasmic reticulum to control mitochondrial function Authors - Wonyul Jang View Article

Reviews

4.8 out of 5

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9 verified customer reviews

What customers say

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Hua (Verified Customer) (07-08-2026)	Great antibody for WB. Applications: Western Blot Primary Antibody Dilution: 1:4000 Cell Tissue Type: HepG2, mouse liver and mouse brain. GRP78/BIP Antibody Western Blot validation (1:4000 dilution) in HepG2, mouse liver and mouse brain. (Cat no:11587-1-AP)
Melina (Verified Customer) (04-03-2026)	Worked well for western of HEK293T cell lysates Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: HEK293T cells GRP78/BIP Antibody Western Blot validation (1:1000 dilution) in HEK293T cells (Cat no:11587-1-AP)
PK (Verified Customer) (03-22-2022)	Applications: Western Blot Cell Tissue Type: FIBROBLAST GRP78/BIP Antibody Western Blot validation ( dilution) in FIBROBLAST (Cat no:11587-1-AP)
Yeawon (Verified Customer) (03-17-2022)	Works very good in mouse kidney tissue. Applications: Western Blot, Immunofluorescence Primary Antibody Dilution: 1:2000 Cell Tissue Type: Mouse kidney tissue
Emma (Verified Customer) (03-15-2022)	Works well by WB in LNCaP and CWR22Rv1 cells @ 1:1000. A single band observed. Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: Prostate
P (Verified Customer) (05-27-2021)	Excellent. Applications: Western Blot Primary Antibody Dilution: 1:2000 Cell Tissue Type: H9C2 GRP78/BIP Antibody Western Blot validation (1:2000 dilution) in H9C2 (Cat no:11587-1-AP)
Kevin (Verified Customer) (01-24-2019)	Works pretty good in HepG2 cells. One single band was observed. Applications: Western Blot, Primary Antibody Dilution: 1:1000 Cell Tissue Type: HepG2
Sylvain (Verified Customer) (12-03-2018)	Applications: Immunofluorescence, Primary Antibody Dilution: 1:300 Cell Tissue Type: colon cancer cell lines
Natalie (Verified Customer) (11-20-2018)	Applications: Western Blot, Primary Antibody Dilution: 1:5000 Cell Tissue Type: Rat Dorsal Root Ganglia GRP78/BIP Antibody Western Blot, validation (1:5000 dilution) in Rat Dorsal Root Ganglia (Cat no:11587-1-AP)