- Featured Product
- KD/KO Validated
GRP78/BIP Polyclonal antibody
GRP78/BIP Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 11587-1-AP
|Positive WB detected in||HEK-293 cells|
|Positive IP detected in||MCF-7 cells|
|Positive IHC detected in||mouse kidney tissue, human breast cancer tissue, human pancreas cancer tissue, mouse heart tissue, rat brain tissue, human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11587-1-AP targets GRP78/BIP in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||Bovine, chicken, cow, Hamster, human, mouse, porcine, rat, sika deer, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GRP78/BIP fusion protein Ag2188|
|Full Name||heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)|
|Calculated molecular weight||78 kDa|
|Observed molecular weight||70-78 kDa|
|GenBank accession number||BC020235|
|Gene ID (NCBI)||3309|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER).It is a constitutively expressed resident protein of the ER in all eukaryotic cells. Recently it has been reported that GRP78 is associated with apoptosis or inhibition of cancer cell growth. 11587-1-AP antibody recognizes one or two bands with MW 70-78 kDa for the signal peptide of GRP78 protein can be finally cleaved.
|Product Specific Protocols|
|WB protocol for GRP78/BIP antibody 11587-1-AP||Download protocol|
|IHC protocol for GRP78/BIP antibody 11587-1-AP||Download protocol|
|IF protocol for GRP78/BIP antibody 11587-1-AP||Download protocol|
|IP protocol for GRP78/BIP antibody 11587-1-AP||Download protocol|
|FC protocol for GRP78/BIP antibody 11587-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Pharmacological preconditioning with the cellular stress inducer thapsigargin protects against experimental sepsis.
Oxid Med Cell Longev
Methane-Rich Saline Ameliorates Sepsis-Induced Acute Kidney Injury through Anti-Inflammation, Antioxidative, and Antiapoptosis Effects by Regulating Endoplasmic Reticulum Stress.
Quantitative proteomic analysis of deer antler stem cells as a model of mammalian organ regeneration.
Porcine circovirus type 2 induces ORF3-independent mitochondrial apoptosis via PERK activation and elevation of cytosolic calcium.
Quantitative interaction proteomics reveals differences in the interactomes of amyloid precursor protein isoforms.
Acta Biochim Biophys Sin (Shanghai)
Chronic intermittent hypoxia accelerates liver fibrosis in rats with combined hypoxia and nonalcoholic steatohepatitis via angiogenesis rather than endoplasmic reticulum stress.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Kevin (Verified Customer) (01-24-2019)
Works pretty good in HepG2 cells. One single band was observed.
Sylvain (Verified Customer) (12-03-2018)
Natalie (Verified Customer) (11-20-2018)