Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 15902-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15902-1-AP (GSTP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15902-1-AP
WB result of GSTP1 antibody (15902-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSTP1 transfected HeLa cells.
WB analysis of human brain using 15902-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 15902-1-AP (GSTP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 15902-1-AP
IP result of anti-GSTP1 (IP:15902-1-AP, 3ug; Detection:15902-1-AP 1:800) with mouse brain tissue lysate 4000ug.
IHC staining of human skin using 15902-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 15902-1-AP (GSTP1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of mouse skin using 15902-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 15902-1-AP (GSTP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skin using 15902-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 15902-1-AP (GSTP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 15902-1-AP
Immunohistochemical analysis of paraffin-embedded human colon slide using 15902-1-AP (GSTP1 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 15902-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 15902-1-AP (GSTP1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 15902-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 15902-1-AP (GSTP1 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HepG2 using 15902-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GSTP1 antibody (15902-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
FC experiment of HepG2 using 15902-1-AP
1x10^6 HepG2 cells were intracellularly stained with 0.4 ug GSTP1 Polyclonal antibody (15902-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO Validated

GSTP1 Polyclonal antibody

Name: GSTP1 Polyclonal antibody
Brand: Proteintech
SKU: 15902-1-AP
Rating: 5.00 (1 reviews)

GSTP1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 15902-1-AP

Publications(38)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Synonyms

GST3/GST pi, GST3, Glutathione S-transferase P, FAEES3, EC:2.5.1.18

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 555 CoraLite® Plus 647

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (38)
Protocols
Reviews (1)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	COLO 320 cells, human brain tissue, HeLa cells, HEK-293 cells, K-562 cells, Jurkat cells, PC-3 cells, mouse brain tissue, mouse heart tissue, rat brain tissue, rat heart tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	mouse skin tissue, human colon, human liver tissue, human skin tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:1600
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 29 publications below
IHC	See 7 publications below
IF	See 7 publications below
IP	See 1 publications below
CoIP	See 1 publications below

Product Information

15902-1-AP targets GSTP1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	GSTP1 fusion protein Ag8731 Predict reactive species
Full Name	glutathione S-transferase pi 1
Calculated Molecular Weight	210 aa, 23 kDa
Observed Molecular Weight	23-28 kDa
GenBank Accession Number	BC010915
Gene Symbol	GSTP1
Gene ID (NCBI)	2950
RRID	AB_2116216
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P09211
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glutathione S-transferase P (GSTP1) conjugates reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. It is involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2) (PubMed:9084911). Participates in the formation of novel hepoxilin regioisomers (PubMed:21046276).

Protocols

Product Specific Protocols
WB protocol for GSTP1 antibody 15902-1-AP	Download protocol
IHC protocol for GSTP1 antibody 15902-1-AP	Download protocol
IF protocol for GSTP1 antibody 15902-1-AP	Download protocol
IP protocol for GSTP1 antibody 15902-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IP	Mol Cell SMURF2 predisposes cancer cell toward ferroptosis in GPX4-independent manners by promoting GSTP1 degradation Authors - Wenxin Zhang KD Validated View Article
mouse	WB	Redox Biol GSTP1-mediated S-glutathionylation of Pik3r1 is a redox hub that inhibits osteoclastogenesis through regulating autophagic flux Authors - Xiaoxiao Ji KD Validated View Article
mouse	IF	J Cell Biol TDP-43 mediates SREBF2-regulated gene expression required for oligodendrocyte myelination. Authors - Wan Yun Ho View Article
mouse	WB	Part Fibre Toxicol Protein target identification and toxicological mechanism investigation of silver nanoparticles-induced hepatotoxicity by integrating proteomic and metallomic strategies. Authors - Ming Xu View Article
mouse	WB	Glia Developmental maturation and regional heterogeneity but no sexual dimorphism of the murine CNS myelin proteome Authors - Sophie B Siems View Article
mouse	WB	Cell Death Dis Poly(ADP-ribosyl)ated PXR is a critical regulator of acetaminophen-induced hepatotoxicity. Authors - Cheng Wang View Article