GSTP1 Polyclonal antibody

Name: GSTP1 Rabbit Polyclonal antibody
Brand: Proteintech
SKU: 15902-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 15902-1-AP

Synonyms

DFN7, FAEES3, Glutathione S transferase P, glutathione S transferase pi 1, GST class pi, GST3, GSTP1, GSTP1 1, PI

WB analysis using 15902-1-AP
Various samples were subjected to SDS PAGE followed by western blot with 15902-1-AP (GSTP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human skin using 15902-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 15902-1-AP (GSTP1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human skin using 15902-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 15902-1-AP (GSTP1 antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HepG2 using 15902-1-AP
Immunofluorescent analysis of HepG2 cells using 15902-1-AP (GSTP1 Antibody) at dilution of 1:50 and Rhodamine-labeled goat anti-rabbit IgG (red).
WB analysis of HeLa using 15902-1-AP
WB result of GSTP1 antibody (15902-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSTP1 transfected HeLa cells.
IP experiment of mouse brain using 15902-1-AP
IP Result of anti-GSTP1 (IP:15902-1-AP, 3ug; Detection:15902-1-AP 1:800) with mouse brain tissue lysate 4000ug.
FC experiment of HepG2 using 15902-1-AP
1X10^6 HepG2 cells were stained with 0.2ug GSTP1 antibody (15902-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Cat No. 15902-1-AP

Freight/Packing: -

Country/Region

Size/Concentration

Quantity

Positive WB detected in	COLO 320 cells, mouse brain tissue, Jurkat cells, PC-3 cells, K-562 cells, human brain tissue, rat brain tissue, HeLa cells
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human skin tissue, human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	HepG2 cells
Positive FC detected in	HepG2 cells

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)	IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

WB	See 12 publications below
IHC	See 2 publications below

15902-1-AP targets GSTP1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	GSTP1 fusion protein Ag8731
Full Name	glutathione S-transferase pi 1
Calculated molecular weight	210 aa, 23 kDa
Observed molecular weight	23-28 kDa
GenBank accession number	BC010915
Gene symbol	GSTP1
Gene ID (NCBI)	2950
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Product Specific Protocols
WB protocol for GSTP1 antibody 15902-1-AP	Download protocol
IHC protocol for GSTP1 antibody 15902-1-AP	Download protocol
IF protocol for GSTP1 antibody 15902-1-AP	Download protocol
IP protocol for GSTP1 antibody 15902-1-AP	Download protocol
FC protocol for GSTP1 antibody 15902-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Species	Application	Title
mouse	WB	J Toxicol Pathol Thioacetamide-induced Hepatocellular Necrosis Is Attenuated in Diet-induced Obese Mice. Authors - Shirai Makoto M View Article
mouse	WB	Biochem Biophys Res Commun Proteomic profiling of brain cortex tissues in a Tau transgenic mouse model of Alzheimer's disease. Authors - Seong-Hun Chang View Article
human	WB	Drug Des Devel Ther MicroRNA-133b targets glutathione S-transferase π expression to increase ovarian cancer cell sensitivity to chemotherapy drugs. Authors - Shuo Chen View Article
human	WB	Food Chem Toxicol Protection of cyanidin-3-glucoside against oxidative stress induced by acrylamide in human MDA-MB-231 cells. Authors - Song Jian J View Article
human	IHC	J Cancer In Vitro and in Vivo Efficacy of NBDHEX on Gefitinib-resistant Human Non-small Cell Lung Cancer. Authors - Huanhuan Sha View Article
mouse	WB	PLoS One Quantitative proteomic analysis of Niemann-Pick disease, type C1 cerebellum identifies protein biomarkers and provides pathological insight. Authors - Cologna Stephanie M SM View Article