Glucagon Monoclonal antibody

Glucagon Monoclonal Antibody for IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2A8G1

Cat no : 67286-1-Ig

Synonyms

GCG, GLP 2, GLP1, GLP2, glucagon, GRPP, GRPP Oxyntomodulin, OXM



Tested Applications

Positive IHC detected inhuman pancreas tissue, mouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman pancreas tissue
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:2000-1:15000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

67286-1-Ig targets Glucagon in IHC, IF, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Glucagon fusion protein Ag10629
Full Name glucagon
Calculated molecular weight 180 aa, 21 kDa
GenBank accession numberBC005278
Gene symbol GCG
Gene ID (NCBI) 2641
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Glucagon is a 29-amino acid peptide hormone secreted from the pancreatic alpha cells with a powerful stimulatory effect on hepatic glucose production acting to increase plasma glucose levels. Glucagon is best known as the counter-regulatory hormone to INS, and normal glucose homeostasis depends largely on the balanced secretion of INS and glucagon from the pancreatic beta and alpha cells, respectively.The regulation of glucose metabolism by glucagon is mediated by its direct action on the peripheral tissues such as the liver and also by the brain. Glucagon is also released postprandially in a transient manner, which was shown to be involved in inhibition of food intake via reduction of meal size.

Protocols

Product Specific Protocols
IHC protocol for Glucagon antibody 67286-1-IgDownload protocol
IF protocol for Glucagon antibody 67286-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols