"HA Tag Antibodies" Comparison
View side-by-side comparison of HA Tag antibodies from other vendors to find the one that best suits your research needs.
Tested Applications
| Positive WB detected in | Transfected HEK-293 cells, HEK-293 cells, Ag19172 |
| Positive IP detected in | Transfected HEK-293 cells |
| Positive IF/ICC detected in | Transfected HEK-293 cells |
| Positive FC (Intra) detected in | Transfected HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:10000-1:100000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66006-2-Ig targets HA Tag in WB, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA, PLA applications and shows reactivity with recombinant protein samples.
| Tested Reactivity | recombinant protein |
| Cited Reactivity | human, mouse, rat, recombinant protein |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | HA Tag |
| Calculated Molecular Weight | 1 kDa |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2881490 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | HATAG |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Protein tags are a protein or peptide sequences located either on the C- or N- terminal of the target protein, which can facilitate solubility, detection, purification, localization, and expression of the target protein. The HA tag corresponds to amino acid residues YPYDVPDYA of a surface glycoprotein -human influenza virus hemagglutinin (HA). The HA tag is commonly used for a variety of research applications including chromatin immunoprecipitation, ELISA, flow cytometry, western blotting, immunocytochemistry/immunofluorescence among of others.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for HA Tag antibody 66006-2-Ig | Download protocol |
| IF protocol for HA Tag antibody 66006-2-Ig | Download protocol |
| IP protocol for HA Tag antibody 66006-2-Ig | Download protocol |
| WB protocol for HA Tag antibody 66006-2-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The HA Tag Monoclonal antibody (1F5C6), catalog number 66006-2-Ig, has 526 citations published in journals including Nature, Science, Cell, Cell Metabolism, Nature Communications, Molecular Cell, Autophagy, Cell Death & Differentiation, Theranostics, Advanced Science, Oncogene, and PNAS. Associated research fields encompass cancer biology (breast, liver, lung, prostate, gastric, colorectal, pancreatic), autophagy, ferroptosis, metabolism, viral infections, neurodegeneration, immunology, inflammation, cardiovascular disease, plant biology, and protein modification.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Aberrant Notch-signaling promotes tumor angiogenesis in esophageal squamous-cell carcinoma | ||
J Hematol Oncol METTL16-SENP3-LTF axis confers ferroptosis resistance and facilitates tumorigenesis in hepatocellular carcinoma | ||
Science DNA-protein cross-links promote cGAS-STING-driven premature aging and embryonic lethality. |
Reviews
What customers say
Customer feedback for this HA tag antibody is mixed. Two users rated it 5 stars: one found it worked very well for Western Blot at 1:5000 dilution, and another praised its low background in immunofluorescence on 293T cells at 1:250. However, one user gave 1 star, reporting non-specific labeling in Western Blot on HEK cells at 1:10000. Overall, the antibody performs well for HA tag detection in both WB and IF, though some users may need to optimize dilution to reduce non-specific binding.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Sarah (Verified Customer) (07-21-2026) | Non-specific labelling
|
Sai Sindhura (Verified Customer) (01-08-2026) | HA tag works very well
|
Parijat (Verified Customer) (03-06-2024) | Good antibody for immunofluorescence. Low background.
|
