CoraLite®488-conjugated IDH1 Monoclonal antibody

IDH1 Monoclonal Antibody for IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF, ELISA

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

2A6A2

Cat no : CL488-66197

Synonyms

IDCD, IDH, IDH1, IDP, IDPC, NADP(+) specific ICDH, Oxalosuccinate decarboxylase, PICD



Tested Applications

Positive IF detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66197-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-66197 targets IDH1 in IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IDH1 fusion protein Ag19293
Full Name isocitrate dehydrogenase 1 (NADP+), soluble
Calculated molecular weight 414 aa, 47 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC012846
Gene symbol IDH1
Gene ID (NCBI) 3417
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IDH1, also named as PICD and IDP, belongs to the isocitrate and isopropylmalate dehydrogenases family. It is a common feature of a major subset of primary human brain cancers. It can form a homodimer(PMID:15173171).IDH1 mutation is always heterozygotic and IDH1 functions as a dimer, theoretically there will be 25% each wild type and mutant homo-dimers and 50% hetero-dimers present in the tumor cells(PMID:21079649 ).

Protocols

Product Specific Protocols
IF protocol for CL488 IDH1 antibody CL488-66197Download protocol
Standard Protocols
Click here to view our Standard Protocols