Cytokeratin 18 Polyclonal antibody

Cytokeratin 18 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (4)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10830-1-AP

Synonyms

CK 18, CYK18, Cytokeratin 18, K18, keratin 18, KRT18



Tested Applications

Positive WB detected inHeLa cells, A431 cells, K-562 cells
Positive IP detected inA431 cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue, human pancreas cancer tissue, human prostate cancer tissue, human renal cell carcinoma tissue, human colon cancer tissue, human ovary tumor tissue, human endometrial cancer tissue, human kidney tissue, human lung cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HepG2 cells, mouse olfactory epithelium tissue
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunohistochemistry (IHC)IHC : 1:200-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10830-1-AP targets Cytokeratin 18 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity calves, cow, duck, human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytokeratin 18 fusion protein Ag1260
Full Name keratin 18
Calculated molecular weight 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC000180
Gene symbol KRT18
Gene ID (NCBI) 3875
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT18, also named as CYK18, PIG46 and K18, is the most commonly found members of the intermediate filament gene family. KRT18 is a type I keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 18 antibody 10830-1-APDownload protocol
IHC protocol for Cytokeratin 18 antibody 10830-1-APDownload protocol
IF protocol for Cytokeratin 18 antibody 10830-1-APDownload protocol
IP protocol for Cytokeratin 18 antibody 10830-1-APDownload protocol
FC protocol for Cytokeratin 18 antibody 10830-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Exp Med

Wnt5a induces and maintains prostate cancer cells dormancy in bone.

Authors - Dong Ren
humanIHC

Oncol Lett

Cytokeratin expression in epidermal stem cells in skin adnexal tumors.

Authors - Xiaoqiu Zhou
cowWB

Theriogenology

Thyroid hormone (T3) is involved in inhibiting the proliferation of newborn calf Sertoli cells via the PI3K/Akt signaling pathway in vitro.

Authors - Chen Wang
mouseIHC

Stress

Restraint stress delays endometrial adaptive remodeling during mouse embryo implantation.

Authors - Guanhui Liu
humanIHC

J Oral Biosci

PLAG1 enhances the stemness profiles of acinar cells in normal human salivary glands in a cell type-specific manner.

Authors - Yuriko Goto
mouseIHC

Exp Lung Res

Atrial natriuretic peptide inhibits epithelial-mesenchymal transition (EMT) of bronchial epithelial cells through cGMP/PKG signaling by targeting Smad3 in a murine model of allergic asthma.

Authors - Shuyuan Chu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Parmveer (Verified Customer) (12-02-2019)

Works well for both IHC and IF, but does come with a bit of background

  • Applications: Immunohistochemistry,Immunofluorescence,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Prostate
Cytokeratin 18 Antibody Immunohistochemistry,Immunofluorescence, validation (1:1000 dilution) in Prostate (Cat no:10830-1-AP)