N-cadherin Polyclonal antibody

N-cadherin Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13769-1-AP

Synonyms



Tested Applications

Positive WB detected inHEK-293 cells, C6 cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13769-1-AP targets N-cadherin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen N-cadherin fusion protein Ag4752
Full Name cadherin 2, type 1, N-cadherin (neuronal)
Calculated molecular weight 906 aa, 100 kDa
Observed molecular weight 90 kDa, 140 kDa
GenBank accession numberBC036470
Gene symbol CDH2
Gene ID (NCBI) 1000
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a classical member of the cadherin superfamily which also include E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619).

Protocols

Product Specific Protocols
WB protocol for N-cadherin antibody 13769-1-APDownload protocol
IHC protocol for N-cadherin antibody 13769-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

Cell Mol Biol Lett

Limbal niche cells can reduce the angiogenic potential of cultivated oral mucosal epithelial cells.

Authors - Chao-Ye Duan
humanWB

Aging (Albany NY)

M6A-related bioinformatics analysis reveals that HNRNPC facilitates progression of OSCC via EMT.

Authors - Guang-Zhao Huang
humanWB

Acta Biomater

A Biomaterial Model of Tumor Stromal Microenvironment Promotes Mesenchymal Morphology but not Epithelial to Mesenchymal Transition in Epithelial Cells.

Authors - Joshua S McLane

Biochim Biophys Acta

AKAP-9 promotes colorectal cancer development by regulating Cdc42 interacting protein 4.

Authors - Zhi-Yan Hu
humanWB

Oncol Lett

Inhibition of cell migration by ouabain in the A549 human lung cancer cell line.

Authors - Liu Ning N
humanWB

Mol Med Rep

miR-200c regulates crizotinib-resistant ALK-positive lung cancer cells by reversing epithelial-mesenchymal transition via targeting ZEB1.

Authors - Hai-Xiang Gao