NF-M Monoclonal antibody

NF-M Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2E3B12

Cat no : 66396-1-Ig

Synonyms

160 kDa neurofilament protein, NEF3, NEFM, neurofilament, Neurofilament 3, NF, NF M, NFM



Tested Applications

Positive WB detected inrat brain tissue, rat brain, mouse brain tissue, PC-12 cells
Positive IHC detected inmouse brain tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunohistochemistry (IHC)IHC : 1:200-1:2000
Sample-dependent, check data in validation data gallery

Product Information

66396-1-Ig targets NF-M in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NF-M fusion protein Ag22709
Full Name neurofilament, medium polypeptide
Calculated molecular weight 102 kDa
Observed molecular weight 140 kDa
GenBank accession numberBC002421
Gene symbol NEFM
Gene ID (NCBI) 4741
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NEFM, also named as NEF3 and NFM, belongs to the intermediate filament family. Neurofilaments are the 10 nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M,140-160 kDa and NF-H, 200-220 kDa.

Protocols

Product Specific Protocols
WB protocol for NF-M antibody 66396-1-IgDownload protocol
IHC protocol for NF-M antibody 66396-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols