CoraLite®594-conjugated NF-M Monoclonal antibody

NF-M Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2E3B12

Cat no : CL594-66396

Synonyms

160 kDa neurofilament protein, NEF3, NEFM, Neurofilament 3, NF M, NFM, NF-M

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Validation Data Gallery

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  IF Staining of mouse brain using CL594-66396

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CoraLite®594 NF-M antibody (CL594-66396, Clone: 2E3B12 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IF detected in: mouse brain tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66396 targets NF-M in IF applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: NF-M fusion protein Ag22709
• Full Name: neurofilament, medium polypeptide
• Calculated Molecular Weight: 102 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC002421
• Gene Symbol: NEFM
• Gene ID (NCBI): 4741
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NEFM, also named as NEF3 and NFM, belongs to the intermediate filament family. Neurofilaments are the 10nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M,140-160 kDa and NF-H, 200-220 kDa.