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  • KD/KO Validated

PCM1 Polyclonal antibody

PCM1 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 19856-1-AP

Synonyms

hPCM 1, PCM 1, PCM1, pericentriolar material 1, PTC4



Tested Applications

Positive WB detected inHEK-293 cells, NIH/3T3 cells, HT-1080 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman tonsillitis tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, NIH/3T3 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 19856-1-AP is PCM1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCM1 fusion protein Ag13929
Full Name pericentriolar material 1
Calculated molecular weight 2024 aa, 228 kDa
Observed molecular weight 260-280 kDa
GenBank accession numberBC000453
Gene symbol PCM1
Gene ID (NCBI) 5108
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Pericentriolar material 1 (PCM1) is a component of centriolar satellites, which are electron dense granules scattered around centrosomes (PMID: 10579718). PCM1 is required for centrosome assembly and function. It acts as a scaffold to target several proteins to the centrosome in a dynein motor-dependent manner and regulate microtubular dynamics (PMID:18762586; 12403812). It is also involved in the biogenesis of cilia (PMID: 18772192). Chromosomal aberrations involving PCM1 gene are associated with papillary thyroid carcinomas and a variety of hematological malignancies, including atypical chronic myeloid leukemia and T-cell lymphoma. (PMID: 10980597; 16091753; 15805263; 16424865).

Protocols

Product Specific Protocols
WB protocol for PCM1 antibody 19856-1-APDownload protocol
IHC protocol for PCM1 antibody 19856-1-APDownload protocol
IF protocol for PCM1 antibody 19856-1-APDownload protocol
FC protocol for PCM1 antibody 19856-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

EMBO Rep

Centriolar satellites are required for efficient ciliogenesis and ciliary content regulation.

Authors - Ezgi Odabasi
  • KO Validated
humanWB

Cell Rep

CCDC57 Cooperates with Microtubules and Microcephaly Protein CEP63 and Regulates Centriole Duplication and Mitotic Progression.

Authors - H Kubra Gurkaslar
humanWB,IF

Front Cell Dev Biol

Distinct Roles of TRAPPC8 and TRAPPC12 in Ciliogenesis via Their Interactions With OFD1.

Authors - Caiyun Zhang
humanWB

Sci Rep

Centrosomal and ciliary targeting of CCDC66 requires cooperative action of centriolar satellites, microtubules and molecular motors.

Authors - Deniz Conkar
  • KD Validated
humanWB,IP

J Biol Chem

The X-linked Deubiquitinase USP9X Is an Integral Component of Centrosome.

Authors - Qian Wang
humanIF

J Cell Sci

Centriolar satellite protein CCDC66 interacts with CEP290 and functions in cilium formation and trafficking.

Authors - Deniz Conkar

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Julie (Verified Customer) (01-06-2020)

This antibody works great and reliably with PFA fix & methanol permeabilization.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: primary fibroblasts