Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, C6 cells |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | U-251 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
82960-1-RR targets RAB32 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | c.elegans |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag1450 Product name: Recombinant human RAB32 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-225 aa of BC015061 Sequence: MAGGGAGDPGLGAAAAPAPETREHLFKVLVIGELGVGKTSIIKRYVHQLFSQHYRATIGVDFALKVLNWDSRTLVRLQLWDIAGQERFGNMTRVYYKEAVGAFVVFDISRSSTFEAVLKWKSDLDSKVHLPNGSPIPAVLLANKCDQNKDSSQSPSQVDQFCKEHGFAGWFETSAKDNINIEEAARFLVEKILVNHQSFPNEENDVDKIKLDQETLRAENKSQCC Predict reactive species |
| Full Name | RAB32, member RAS oncogene family |
| Calculated Molecular Weight | 25 kDa |
| Observed Molecular Weight | 25-28 kDa |
| GenBank Accession Number | BC015061 |
| Gene Symbol | RAB32 |
| Gene ID (NCBI) | 10981 |
| RRID | AB_3670705 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q13637 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Rab32 belongs to the Ras-like small GTPase superfamily, which regulate membrane dynamics, including membrane fusion/fission, exocytosis, and cytoskeletal trafficking. Rab32 anchors the type II regulatory subunit of protein kinase A to the mitochondrion and aids in mitochondrial fission. TRab32 also appears to be involved in autophagy and melanosome secretion.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for RAB32 antibody 82960-1-RR | Download protocol |
| IF protocol for RAB32 antibody 82960-1-RR | Download protocol |
| WB protocol for RAB32 antibody 82960-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RAB32 Recombinant monoclonal antibody (Cat# 82960-1-RR) has 1 citation to date. It appears in the Journal of Biological Chemistry (2025), where it was used for Western blot in C. elegans to study FAT-5/SCD5-mediated lipid localization in lysosomes and its role in alleviating gamma radiation injury. The research field encompasses lipid metabolism, lysosomal biology, and radiation biology.
| Species | Application | Title |
|---|---|---|
J Biol Chem FAT-5/SCD5-mediated lipid localization in lysosomes alleviates gamma radiation injury in Caenorhabditis elegans. |
Reviews
What customers say
The single review for 82960-1-RR is highly positive (5/5). The user employed the antibody for Western Blot at a 1:500 dilution in HeLaM cells to assess siRNA knockdown efficiency. They reported that the band size was accurate and the signal intensity was well suited to their application, indicating reliable performance for their experimental needs.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Ioana (Verified Customer) (08-27-2025) | This antibody has helped me calculate the efficiency of siRNA KD in my cell lines . The band size was accurate and the intensity was suitable for my application.
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