Tested Applications
| Positive WB detected in | T-47D cells, HeLa cells, MCF-7 cells, U-251 cells, U2OS cells, human placenta tissue |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83440-2-RR targets TMEM126A in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag14705 Product name: Recombinant human TMEM126A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 67-195 aa of BC007875 Sequence: AGLPMAGIPFLTTDLTYRCFVSFPLNTGDLDCETCTITRSGLTGLVIGGLYPVFLAIPVNGGLAARYQSALLPHKGNILSYWIRTSKPVFRKMLFPILLQTMFSAYLGSEQYKLLIKALQLSEPGKEIH Predict reactive species |
| Full Name | transmembrane protein 126A |
| Calculated Molecular Weight | 195 aa, 22 kDa |
| Observed Molecular Weight | 20-25 kDa |
| GenBank Accession Number | BC007875 |
| Gene Symbol | TMEM126A |
| Gene ID (NCBI) | 84233 |
| RRID | AB_3671081 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9H061 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TMEM126A is a mitochondrial inner membrane protein that is enriched in the cristae. TMEM126A is necessary for Complex I assembly or stability.Complex I (CI) is the largest enzyme of the mitochondrial respiratory chain, and its defects are the main cause of mitochondrial disease.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for TMEM126A antibody 83440-2-RR | Download protocol |
| IF protocol for TMEM126A antibody 83440-2-RR | Download protocol |
| WB protocol for TMEM126A antibody 83440-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Aditya (Verified Customer) (06-16-2025) | did not get any bands in the region specified by proteintech (hek lysate), cut the blot for this, so may have been banding above or below that i didnt see
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