UBE1 Antibody

UBE1 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1H7E8

Cat no : 67198-1-Ig

Synonyms

A1S9, A1S9T, A1ST, AMCX1, GXP1, Protein A1S9, SMAX2, UBA1, UBA1A, UBE1, UBE1X, Ubiquitin activating enzyme E1



Tested Applications

Positive WB detected inHeLa cells, HeLa cell, HEK-293 cells, Jurkat cells, HL-60 cells, K-562 cells, HSC-T6 cells, RAW 264.7 cells, NIH/3T3 cells
Positive IHC detected inhuman colon cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:30000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

67198-1-Ig targets UBE1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen UBE1 fusion protein Ag8920
Full Name ubiquitin-like modifier activating enzyme 1
Calculated molecular weight 1058aa,118 kDa
Observed molecular weight 114-118 kDa
GenBank accession number BC013041
Gene symbol UBA1
Gene ID (NCBI) 7317
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for UBE1 antibody 67198-1-IgDownload protocol
IHC protocol for UBE1 antibody 67198-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols