CoraLite® Plus 488-conjugated UBE1 Monoclonal antibody

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  WB analysis using CL488-67198

  Various lysates were subjected to SDS PAGE followed by western blot with CL488-67198 (UBE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

UBE1 Monoclonal Antibody for WB

Cat No. CL488-67198

Clone No.1H7E8

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB

Synonyms

A1S9, A1S9T, A1ST, AMCX1, GXP1, Protein A1S9, SMAX2, UBA1, UBA1A, UBE1, UBE1X, Ubiquitin activating enzyme E1

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Tested Applications

• Positive WB detected in: HEK-293 cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67198 targets UBE1 in WB applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8920

  Product name: Recombinant human UBE1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 711-1058 aa of BC013041

  Sequence: CHHWHTQYSNNIRQLLHNFPPDQLTSSGAPFWSGPKRCPHPLTFDVNNPLHLDYVMAAANLFAQTYGLTGSQDRAAVATFLQSVQVPEFTPKSGVKIHVSDQELQSANASVDDSRLEELKATLPSPDKLPGFKMYPIDFEKDDDSNFHMDFIVAASNLRAENYDIPSADRHKSKLIAGKIIPAIATTTAAVVGLVCLELYKVVQGHRQLDSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLKQFLDYFKTEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVSKRKLGRHVRALVLELCCNDESGEDVEVPYVRYTIR

• Full Name: ubiquitin-like modifier activating enzyme 1
• Calculated Molecular Weight: 1058 aa, 118 kDa
• Observed Molecular Weight: 114-118 kDa
• GenBank Accession Number: BC013041
• Gene Symbol: UBA1
• Gene ID (NCBI): 7317
• RRID: AB_3084324
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P22314
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.