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CoraLite594-conjugated alpha Tubulin Antibody 0 Publications

Mouse Monoclonal| Catalog number: CL594-66031 |CloneNo.: 1E4C11

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Con: 100 μg/100 μl

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Tested Applications

Applications:
IF, FC, ELISA
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Images See all

alpha Tubulin Antibody IF HepG2 cells CL594-66031

Immunofluorescent analysis of ( -20°C Ethanol) fixed HepG2 cells using CL594-66031 (alpha Tubulin antibody) at dilution of 1:50 and N/A

alpha Tubulin Antibody FC HepG2 cells CL594-66031

1X10^6 HepG2 cells were stained with 0.20ug alpha Tubulin antibody (CL594-66031, red) and control antibody (blue). Fixed with 90% MeOH

alpha Tubulin Antibody IF HepG2 cells CL594-66031

Immunofluorescent analysis of ( -20°C Ethanol) fixed HepG2 cells using CL594-66031 (alpha Tubulin antibody) at dilution of 1:100.

Species specificity:
human, mouse, rat, dog
Positive IF detected in:
HepG2 cells
Positive FC detected in:
HepG2 cells
Recommended dilution:
IF : 1:50-1:500
Sample-dependent, check data in Validation tab
Excitation/Emission maxima wavelengths:
593 nm/614 nm

Product Information

Source:
Mouse
Purification method:
Protein A purification
Isotype:
IgG2b
Storage:
PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
alpha Tubulin fusion protein Ag18034
Full name:
tubulin, alpha 1b
Calculated molecular weight:
50 kDa
Observed molecular weight:
GenBank accession number:
BC009314
Gene ID (NCBI):
10376
Gene symbol
TUBA1B
Synonyms
Alpha tubulin ubiquitous, alpha tubulin,a tubulin, K ALPHA 1, TUBA1B, Tubulin alpha 1B chain, Tubulin alpha ubiquitous chain, Tubulin K alpha 1, tubulin, alpha 1b

Background

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha tubulin is useful for scientists across fields as an internal control due to its high, ubiquitous expression pattern. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs. This anitbody is CL594(Ex/Em 593 nm/614 nm) conjugated.


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