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CoraLite®594-conjugated Alpha Tubulin Monoclonal antibody

Alpha Tubulin Monoclonal Antibody for FC, IF, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, dog

Applications

WB, IF, FC, ELISA

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1E4C11

Cat no : CL594-66031

Synonyms

Alpha tubulin ubiquitous, alpha tubulin,a tubulin, K ALPHA 1, TUBA1B, Tubulin alpha 1B chain, Tubulin alpha ubiquitous chain, Tubulin K alpha 1, tubulin, alpha 1b, α-tubulin

Validation Data Gallery

  • WB analysis using CL594-66031

    WB analysis using CL594-66031

    Various lysates were subjected to SDS PAGE followed by western blot with CL594-66031 (Alpha Tubulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using CL594-66031

    FC experiment of HepG2 using CL594-66031

    1X10^6 HepG2 cells were stained with 0.20ug alpha Tubulin antibody (CL594-66031, red) and control antibody (blue). Fixed with 90% MeOH.

  • IF Staining of HeLa using CL594-66031

    IF Staining of HeLa using CL594-66031

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using the CoraLite®594-conjugated version of this antibody, CL594-66031 (alpha Tubulin antibody), at dilution of 1:100.

  • IF Staining of HepG2 using CL594-66031

    IF Staining of HepG2 using CL594-66031

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-66031 (alpha Tubulin antibody) at dilution of 1:100.

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HSC-T6 cells, NIH/3T3 cells
Positive IF detected inHepG2 cells, HeLa cells
Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66031-1-Ig

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of CL594-66031 is Alpha Tubulin Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat, dog
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Alpha Tubulin fusion protein Ag18034
Full Name tubulin, alpha 1b
Calculated molecular weight 50 kDa
GenBank accession numberBC009314
Gene symbol TUBA1B
Gene ID (NCBI) 10376
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths593 nm/614 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha tubulin is useful for scientists across fields as an internal control due to its high, ubiquitous expression pattern. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs. This anitbody is CL594(Ex/Em 593 nm/614 nm) conjugated.

Protocols

Product Specific Protocols
WB protocol for CL594 Alpha Tubulin antibody CL594-66031Download protocol
IF protocol for CL594 Alpha Tubulin antibody CL594-66031Download protocol
FC protocol for CL594 Alpha Tubulin antibody CL594-66031Download protocol
Standard Protocols
Click here to view our Standard Protocols