CoraLite®594-conjugated Alpha Tubulin Monoclonal antibody
Alpha Tubulin Monoclonal Antibody for FC, IF, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat, dog
Applications
WB, IF, FC, ELISA
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
1E4C11
Cat no : CL594-66031
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, HEK-293 cells, HSC-T6 cells, NIH/3T3 cells |
Positive IF detected in | HepG2 cells, HeLa cells |
Positive FC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:300-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Product Information
The immunogen of CL594-66031 is Alpha Tubulin Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat, dog |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | Alpha Tubulin fusion protein Ag18034 |
Full Name | tubulin, alpha 1b |
Calculated molecular weight | 50 kDa |
GenBank accession number | BC009314 |
Gene symbol | TUBA1B |
Gene ID (NCBI) | 10376 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 593 nm/614 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha tubulin is useful for scientists across fields as an internal control due to its high, ubiquitous expression pattern. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs. This anitbody is CL594(Ex/Em 593 nm/614 nm) conjugated.
Protocols
Product Specific Protocols | |
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WB protocol for CL594 Alpha Tubulin antibody CL594-66031 | Download protocol |
IF protocol for CL594 Alpha Tubulin antibody CL594-66031 | Download protocol |
FC protocol for CL594 Alpha Tubulin antibody CL594-66031 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |