CD80 Monoklonaler Antikörper

CD80 Monoklonal Antikörper für FC

Wirt / Isotyp

Armenian Hamster / IgG

Getestete Reaktivität

Maus

Anwendung

IF, FC

Konjugation

FITC Plus Fluorescent Dye

Publikationen(17)

CloneNo.

16-10A1

Kat-Nr. : FITC-65076

Synonyme

Ly53, Ly 53, CD80 antigen, Cd28l, B7.1

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  FC experiment of Balb/c mouse peritoneal macrophages using FITC-65076

  1x10^6 Balb/c mouse peritoneal macrophages were surface stained with 0.5 ug FITC Plus Anti-Mouse CD80 (B7-1) (16-10A1) (FITC-65076, Clone: 16-10A1) (red), or 0.5 ug FITC Armenian Hamster IgG Isotype Control (PIP) (FITC-65210, Clone: PIP) (blue). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Balb/c peritoneale Makrophagen der Maus

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 2 publications below
• FC: See 14 publications below

Produktinformation

FITC-65076 bindet in IF, FC CD80 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Armenian Hamster / IgG
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CHO-Zelllinie transfiziert mit Maus-B7 (CD80)
• Vollständiger Name: CD80 antigen
• GenBank-Zugangsnummer: BC131959
• Gene symbol: CD80
• Gene ID (NCBI): 12519
• Konjugation: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)

Publikationen

Species	Application	Title
mouse	FC	ACS Appl Mater Interfaces; Combined Chemo-Immuno-Photothermal Therapy for Effective Cancer Treatment via an All-in-One and One-for-All Nanoplatform; Authors - Xianglong Yu
mouse	FC	ACS Appl Mater Interfaces; Intelligent Nanoplatform Integrating Macrophage and Cancer Cell Membrane for Synergistic Chemodynamic/Immunotherapy/Photothermal Therapy of Breast Cancer; Authors - Shichao Zhang
mouse	IF	Cell Rep; Mechanotransduction in response to ECM stiffening impairs cGAS immune signaling in tumor cells; Authors - Yingqi Liu
mouse	FC	ACS Appl Mater Interfaces; Dual-Regulated Biomimetic Nanocomposites For Promoted Tumor Photodynamic Immunotherapy; Authors - Li Liao
mouse	FC	Neurobiol Dis; Inhibitory effects of xanthine oxidase inhibitor, topiroxostat, on development of neuropathy in db/db mice.; Authors - Kazuhisa Takahashi
	FC	Nat Commun; CD36-mediated metabolic crosstalk between tumor cells and macrophages affects liver metastasis; Authors - Ping Yang