IBA1 Polyklonaler Antikörper

IBA1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(14)

Kat-Nr. : 26177-1-AP

Synonyme

AIF1, AIF 1, IBA 1, G1, allograft inflammatory factor 1

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  WB analysis of THP-1 using 26177-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 26177-1-AP (IBA1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of rat brain using 26177-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 26177-1-AP (IBA1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 26177-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 26177-1-AP (IBA1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 26177-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26177-1-AP (IBA1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 26177-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26177-1-AP (IBA1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: THP-1-Zellen
• Erfolgreiche Detektion in IHC: Rattenhirngewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:400-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 4 publications below
• IF: See 7 publications below

Produktinformation

26177-1-AP bindet in WB, IHC, IF, ELISA IBA1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IBA1 fusion protein Ag24000
• Vollständiger Name: allograft inflammatory factor 1
• Berechnetes Molekulargewicht: 17 kDa
• GenBank-Zugangsnummer: BC009474
• Gene symbol: IBA1
• Gene ID (NCBI): 199
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IBA1 is a 143 amino acid cytoplasmic, inflammation response scaffold protein. It is constitutively expressed in monocytes and macrophages and is known to be involved in macrophage activation. It is a marker of activated macrophage. Expression of IBA1 is up-regulated in activated microglia following facial nerve axotomy, ischemia, and several brain diseases, thereby implicating it in the activated phenotypes of microglia.

Publikationen

Species	Application	Title
mouse	WB	Alzheimers Res Ther; Circular RNA APP contributes to Alzheimer's disease pathogenesis by modulating microglial polarization via miR-1906/CLIC1 axis; Authors - Deng-Pan Wu
mouse	IF	Fitoterapia; LC-MS/MS analysis of Shenghui decoction component and its effect on learning and memory and neuroprotection in sleep deprivation model mice; Authors - Chun Wen
mouse,rat	WB,IF	Toxicon; Loganin reduces neuroinflammation and promotes functional recovery after spinal cord injury through inhibiting NF-κB/NLRP3 signalling; Authors - Xudong Huang
mouse	WB	Front Pharmacol; Shikimic acid (SA) inhibits neuro-inflammation and exerts neuroprotective effects in an LPS-induced in vitro and in vivo model; Authors - Xueying Bao
rat	IHC	J Pain Res; Rat Model of Neuropathic Pain Induced by Spinal Nerve Ligation: A New Approach via an Oblique Lateral Incision; Authors - Zhihong Cheng
mouse	WB	Brain Res Bull; Induction of tau pathology and motor dysfunction in mice by urinary exosomes from progressive supranuclear palsy patients; Authors - Qiusha Li