Ly-6G Monoklonaler Antikörper

Ly-6G Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG2a, kappa

Getestete Reaktivität

Maus

Anwendung

IF, FC

Konjugation

FITC Plus Fluorescent Dye

Publikationen(1)

CloneNo.

1A8

Kat-Nr. : FITC-65078

Synonyme

Ly6g, Ly-6G.1, Ly 6G, 1A8

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  FC experiment of mouse bone marrow cells using FITC-65078

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug FITC Anti-Mouse Ly-6G (1A8) (FITC-65078, Clone:1A8) or FITC Plus Rat IgG2a Isotype Control (2A3) (FITC-65209, Clone: 2A3). Cells were not fixed.

• 

  FC experiment of mouse bone marrow cells using FITC-65078

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug FITC Anti-Mouse Ly-6G (1A8) (FITC-65078, Clone:1A8) (red) or FITC Plus Rat IgG2a Isotype Control (2A3) (FITC-65209, Clone: 2A3) (blue). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Knochenmarkzellen

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

FITC-65078 bindet in IF, FC Ly-6G und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2a, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Ly-6G-transfizierte EL-4J-Zelllinie
• Vollständiger Name: lymphocyte antigen 6 complex, locus G
• GenBank-Zugangsnummer: X70920
• Gene symbol: Ly-6G
• Gene ID (NCBI): 546644
• Konjugation: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Ly-6G (lymphocyte antigen 6 complex, locus G), also known as Gr-1, is a 21-25 kDa, glycosylphosphatidylinositol-anchored protein expressed on myeloid lineage cells in mouse bone marrow (PMID: 8360469). The expression of Ly-6G increases on neutrophils as they differentiate from immature cells in the bone marrow to mature cells in the blood and spleen (PMID: 8890901). Antibodies targeting Ly6G (RB6-8C5 or 1A8) are commonly used in studies aimed at identifying the role of neutrophils (PMID: 23543767). The 1A8 mAb is specific for Ly-6G (PMID: 8360469).

Publikationen

Species	Application	Title
mouse	IF	J Tradit Complement Med; Unveiling pharmacological targets of Rihimaside C for radiation-induced lung injury: An in silico and experimental integrated approach; Authors - Youyi Liu