FC experiment of mouse bone marrow cells using Biotin-98284
1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Biotin Anti-Mouse Ly-6G Rabbit RecAb (Biotin-98284, Clone: 242141B11) and CoraLite®488-conjugated streptavidin, or unstained. Cells were co-stained with APC Anti-Mouse CD11b (M1/70) (APC-65055, Clone: M1/70). Cells were not fixed.
1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Biotin Anti-Mouse Ly-6G Rabbit RecAb (Biotin-98284, Clone: 242141B11) and CoraLite®488-conjugated streptavidin, or unstained. Cells were co-stained with APC Anti-Mouse CD11b (M1/70) (APC-65055, Clone: M1/70). Cells were not fixed.
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This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
Biotin-98284 targets Ly-6G in FC applications and shows reactivity with mouse samples.
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
Background Information
Ly-6G (lymphocyte antigen 6 complex, locus G), also known as Gr-1, is a 21-25 kDa, glycosylphosphatidylinositol-anchored protein expressed on myeloid lineage cells in mouse bone marrow (PMID: 8360469). The expression of Ly-6G increases on neutrophils as they differentiate from immature cells in the bone marrow to mature cells in the blood and spleen (PMID: 8890901).
Protocols
Product Specific Protocols
FC protocol for Biotin Ly-6G antibody Biotin-98284
FC experiment of mouse bone marrow cells using Biotin-98284
1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Biotin Anti-Mouse Ly-6G Rabbit RecAb (Biotin-98284, Clone: 242141B11) and CoraLite®488-conjugated streptavidin, or unstained. Cells were co-stained with APC Anti-Mouse CD11b (M1/70) (APC-65055, Clone: M1/70). Cells were not fixed.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
Ly-6G Recombinant antibody
Catalog Number
Biotin-98284
Citations
-
Dilutions
Applications
FC
Reactivity
mouse
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
