Anticorps Monoclonal anti-CD68

CD68 Monoclonal Antibody for FC (Intra)

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain

Applications

FC (Intra)

Conjugaison

FITC Plus Fluorescent Dye

Publications(3)

CloneNo.

KP1

N° de cat : FITC-65202

Synonymes

SCARD1, Macrosialin, KP1, GP110, CD68 molecule

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Galerie de données de validation

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  FC experiment of human PBMCs using FITC-65202

  1X10^6 human PBMCs were intracellularly stained with 5 ul FITC Plus Anti-Human CD68 (FITC-65202, Clone: KP1) (red), or Mouse IgG1 Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

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  FC experiment of human PBMCs using FITC-65202

  1X10^6 human PBMCs were intracellularly stained with 5 ul FITC Plus Anti-Human CD68 (FITC-65202, Clone: KP1) (right), or Mouse IgG1 Isotype Control (left). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

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Applications testées

• Résultats positifs en FC (Intra): CMSP humaines

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Applications publiées

• FC: See 2 publications below

Informations sur le produit

FITC-65202 cible CD68 dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Lysosomal fraction of human alveolar macrophages
• Nom complet: CD68 molecule
• Masse moléculaire calculée: 37 kDa
• Numéro d’acquisition GenBank: BC015557
• Symbole du gène: CD68
• Identification du gène (NCBI): 968
• Conjugaison: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.

Publications

Species	Application	Title
human	FC	Cancer Immunol Immunother; SUMO modified ETV1 promotes M2-polarized tumor-associated macrophage infiltration and cancer progression by facilitating CCL2 transcription in esophageal squamous cell carcinoma cells; Authors - Tianci Han
human	FC (Intra)	Front Bioeng Biotechnol; Evaluation of nanoparticle albumin-bound paclitaxel loaded macrophages for glioblastoma treatment based on a microfluidic chip; Authors - Zuorun Xie
	FC	Int Immunopharmacol; FOXF2 suppressed esophageal squamous cell carcinoma by reducing M2 TAMs via modulating RNF144A-FTO axis; Authors - Tianci Han

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