Anticorps Monoclonal anti-CD80

CD80 Monoclonal Antibody for FC

Hôte / Isotype

Armenian Hamster / IgG

Réactivité testée

souris

Applications

IF, FC

Conjugaison

FITC Plus Fluorescent Dye

Publications(18)

CloneNo.

16-10A1

N° de cat : FITC-65076

Synonymes

Ly53, Ly 53, CD80 antigen, Cd28l, B7.1

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Galerie de données de validation

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  FC experiment of Balb/c mouse peritoneal macrophages using FITC-65076

  1x10^6 Balb/c mouse peritoneal macrophages were surface stained with 0.5 ug FITC Plus Anti-Mouse CD80 (B7-1) (16-10A1) (FITC-65076, Clone: 16-10A1) (red), or 0.5 ug FITC Armenian Hamster IgG Isotype Control (PIP) (FITC-65210, Clone: PIP) (blue). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: macrophages péritonéaux de souris Balb/c,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 2 publications below
• FC: See 15 publications below

Informations sur le produit

FITC-65076 cible CD80 dans les applications de IF, FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: souris
• Hôte / Isotype: Armenian Hamster / IgG
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Lignée de cellules CHO transfectées avec B7 (CD80) de souris
• Nom complet: CD80 antigen
• Numéro d’acquisition GenBank: BC131959
• Symbole du gène: CD80
• Identification du gène (NCBI): 12519
• Conjugaison: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)

Publications

Species	Application	Title
mouse	FC	ACS Appl Mater Interfaces; Combined Chemo-Immuno-Photothermal Therapy for Effective Cancer Treatment via an All-in-One and One-for-All Nanoplatform; Authors - Xianglong Yu
mouse	FC	ACS Appl Mater Interfaces; Intelligent Nanoplatform Integrating Macrophage and Cancer Cell Membrane for Synergistic Chemodynamic/Immunotherapy/Photothermal Therapy of Breast Cancer; Authors - Shichao Zhang
mouse	IF	Cell Rep; Mechanotransduction in response to ECM stiffening impairs cGAS immune signaling in tumor cells; Authors - Yingqi Liu
mouse	FC	ACS Appl Mater Interfaces; Dual-Regulated Biomimetic Nanocomposites For Promoted Tumor Photodynamic Immunotherapy; Authors - Li Liao
mouse	FC	Neurobiol Dis; Inhibitory effects of xanthine oxidase inhibitor, topiroxostat, on development of neuropathy in db/db mice.; Authors - Kazuhisa Takahashi
	FC	Nat Commun; CD36-mediated metabolic crosstalk between tumor cells and macrophages affects liver metastasis; Authors - Ping Yang

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