Anticorps Polyclonal de lapin anti-human IgG heavy chain

human IgG heavy chain Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, RIP, IP, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

12

Avis (1)

N° de cat : 16402-1-AP

Synonymes

human IgG heavy chain, IgG Fc, IGHG, IGHG4, Immunoglobulin heavy chain gamma

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Galerie de données de validation

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  WB analysis of human plasma using 16402-1-AP

  human plasma (0.3ug) were subjected to SDS PAGE followed by western blot with 16402-1-AP (human IgG heavy chain Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 16402-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16402-1-AP (human IgG heavy chain antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 16402-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16402-1-AP (human IgG heavy chain antibody) at dilution of 1:200 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 16402-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16402-1-AP (human IgG heavy chain antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu plasmatique humain, cellules Jurkat
• Résultats positifs en IHC: tissu d'amygdalite humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:20000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 2 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below
• RIP: See 2 publications below

Informations sur le produit

16402-1-AP cible human IgG heavy chain dans les applications de WB, RIP, IP, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: human IgG heavy chain Protéine recombinante Ag9519
• Nom complet: immunoglobulin heavy constant gamma 4 (G4m marker)
• Masse moléculaire calculée: 473 aa, 52 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC025985
• Symbole du gène: IGHG4
• Identification du gène (NCBI): 3503
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IGHG4 has some fragments with MW 36 kDa (http://www.uniprot.org/uniprot/P01861) and 52 kDa (http://www.ncbi.nlm.nih.gov/protein/CAE45900). This antibody detects all the Heavy chain of human immunoglobulin gamma (IgG), IgG1, IgG2, IgG3, IgG4 included.

Publications

Species	Application	Title
human	IHC	Nat Commun; Cerebrospinal fluid lipocalin 2 as a novel biomarker for the differential diagnosis of vascular dementia.; Authors - Franc Llorens
human	IHC	Neuropathol Appl Neurobiol; Plasma total prion protein as a potential biomarker for neurodegenerative dementia: diagnostic accuracy in the spectrum of prion diseases.; Authors - Franc Llorens
human	CoIP	Mol Oncol; Secretogranin II impairs tumor growth and angiogenesis by promoting degradation of hypoxia-inducible factor-1α in colorectal cancer.; Authors - Chao Fang
	WB	Oncol Rep; Upregulation of IGF1 by tumor-associated macrophages promotes the proliferation and migration of epithelial ovarian cancer cells.; Authors - Li Liu
		Int J Mol Sci; Identification of Potential Biomarkers for Rhegmatogenous Retinal Detachment Associated with Choroidal Detachment by Vitreous iTRAQ-Based Proteomic Profiling.; Authors - Zhifeng Wu
	RIP	Front Genet; A Circ-0007022/miR-338-3p/Neuropilin-1 Axis Reduces the Radiosensitivity of Esophageal Squamous Cell Carcinoma by Activating Epithelial-To-Mesenchymal Transition and PI3K/AKT Pathway.; Authors - Junpeng Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Pratheepa (Verified Customer) (06-11-2021)

The antibody works excellent. But the specificity is not restricted to human cells. It picks up mouse cell line also.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Adipose stem cell, Mouse microglial cell

human IgG heavy chain Antibody Immunofluorescence validation (1:200 dilution) in Adipose stem cell, Mouse microglial cell (Cat no:16402-1-AP)