Tested Applications
| Positive WB detected in | A549 cells, HEK-293 cells, HeLa cells, human spleen tissue, MCF-7 cells, Calu-1 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, MDCK cells, HEK-293 cells, HeLa cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11308-1-AP targets GOLGA2/GM130 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, canine samples.
| Tested Reactivity | human, canine |
| Cited Reactivity | human, pig, monkey, zebrafish, hamster, sheep |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1848 Product name: Recombinant human GM130;GOLGA2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 361-678 aa of BC014188 Sequence: LRAQLSLMAHPGEGDGLDREEEEDEEEEEEEAVAVPQPMPSIPEDLESREAMVAFFNSAVASAEEEQARLRGQLKEQRVRCRRLAHLLASAQKEPEAAAPAPGTGGDSVCGETHRALQGAMEKLQSRFMELMQEKADLKERVEELEHRCIQLSGETDTIGEYIALYQSQRAVLKERHREKEEYISRLAQDKEEMKVKLLELQELVLRLVGDRNEWHGRFLAAAQNPADEPTSGAPAPQELGAANQQGDLCEVSLAGSVEPAQGEAREGSPRDNPTAQQIMQLLREMQNPRERPGLGSNPCIPFFYRADENDEVKITVI Predict reactive species |
| Full Name | golgi autoantigen, golgin subfamily a, 2 |
| Calculated Molecular Weight | 111 kDa |
| Observed Molecular Weight | 130 kDa |
| GenBank Accession Number | BC014188 |
| Gene Symbol | GM130 |
| Gene ID (NCBI) | 2801 |
| RRID | AB_2115327 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q08379 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GOLGA2, also known as GM130, is a 130 kDa cis-Golgi matrix protein which is one component of the detergent and salt resistant Golgi matrix. It is a peripheral membrane protein highly bound to Golgi membrane and localized mainly at the cytoplasmic face of cis-Golgi membrane. Together with its interacting partner proteins, including p115, giantin, GRASP65, and Rab GTPase, GOLGA2/GM130 is involved in the regulation of ER-to-Golgi transport and also in the maintenance of the Golgi structure. Emerging evidence suggest that the GOLGA2/GM130 has potential roles in the control of glycosylation, cell cycle progression, and higher order cell functions such as cell polarization and directed cell migration. (PMID: 20197635)
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for GOLGA2/GM130 antibody 11308-1-AP | Download protocol |
| IF protocol for GOLGA2/GM130 antibody 11308-1-AP | Download protocol |
| IHC protocol for GOLGA2/GM130 antibody 11308-1-AP | Download protocol |
| IP protocol for GOLGA2/GM130 antibody 11308-1-AP | Download protocol |
| WB protocol for GOLGA2/GM130 antibody 11308-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GOLGA2/GM130 Polyclonal antibody (Cat# 11308-1-AP) has accumulated 243 citations. Publications span high-impact journals including Nature, Cell, Cell Metabolism, Immunity, Nature Cell Biology, Nature Communications, EMBO Journal, Molecular Cell, Science Advances, Autophagy, Cell Reports, and Journal of Clinical Investigation. Key research fields include Golgi apparatus biology, lipid metabolism, autophagy, extracellular vesicle/exosome biology, cancer biology, viral infections, innate immunity, ferroptosis, neurodegeneration, and tissue regeneration.
| Species | Application | Title |
|---|---|---|
Cell Unraveling cellular complexity with transient adapters in highly multiplexed super-resolution imaging |
Reviews
What customers say
All three reviews rate the antibody 5 stars. Users report excellent performance in both Western blot and immunofluorescence applications. For IF, one user achieved a very strong, specific Golgi signal in SH-SY5Y cells at 1:1000 dilution, while another successfully co-stained GM130 with EEA1 and DAPI in human RPE cells at 1:300. The WB user described the result as "very good." Overall, reviewers highlight strong specificity, clear Golgi localization, and reliable performance across cell types and applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Tatyana (Verified Customer) (06-15-2025) | Incubation at RT for 1 h in 5% goat serum/PBST. Very strong specific signal of Golgi apparatus.
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Boyan (Verified Customer) (04-02-2023) | very good for WB.
|
Stephen (Verified Customer) (10-01-2019) | RPE cells fixed with 4% PFA Perm. by 0.3% tx100 for 5 min blocked with 1% BSA in 1XPBS for 2 hours GM130 antibody (green) incubated 1:300 and EEA1 antibody (purple) overnight at 4 degrees in 1%BSA in 1x PBS. Co-stained with DAPI (blue) visualize DNA
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