Tested Applications
| Positive WB detected in | mouse skeletal muscle tissue, human heart tissue, human skeletal muscle tissue, mouse heart tissue, MCF-7 cells, rat skeletal muscle tissue |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11590-1-AP targets RGS5 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2196 Product name: Recombinant human RGS5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-181 aa of BC030059 Sequence: MCKGLAALPHSCLERAKEIKIKLGILLQKPDSVGDLVIPYNEKPEKPAKTQKTSLDEALQWRDSLDKLLQNNYGLASFKSFLKSEFSEENLEFWIACEDYKKIKSPAKMAEKAKQIYEEFIQTEAPKEVNIDHFTKDITMKNLVEPSLSSFDMAQKRIHALMEKDSLPRFVRSEFYQELIK Predict reactive species |
| Full Name | regulator of G-protein signaling 5 |
| Calculated Molecular Weight | 181 aa, 21 kDa |
| Observed Molecular Weight | 42 kDa |
| GenBank Accession Number | BC030059 |
| Gene Symbol | RGS5 |
| Gene ID (NCBI) | 8490 |
| RRID | AB_10644290 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15539 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RGS5 inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. It binds to G(i)-alpha and G(o)-alpha, but not to G(s)-alpha. The 42kDa band recognized by this antibody is a RGS5-RGS5 dimer. (Pubmed:17762159)
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for RGS5 antibody 11590-1-AP | Download protocol |
| IF protocol for RGS5 antibody 11590-1-AP | Download protocol |
| IHC protocol for RGS5 antibody 11590-1-AP | Download protocol |
| WB protocol for RGS5 antibody 11590-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RGS5 polyclonal antibody (Cat# 11590-1-AP) has 25 citations across journals including Cell, Nature Communications, Cancer Research, Advanced Science, Cell Reports, Communications Biology, Translational Oncology, PLoS ONE, and others. Research fields span tumor microenvironment and cancer-associated fibroblasts, single-cell transcriptomics, vascular smooth muscle biology, preeclampsia, NAFLD/NASH, wound healing, and viral pathogenesis.
| Species | Application | Title |
|---|---|---|
Cell Beta-Arrestin1 Prevents Preeclampsia by Downregulation of Mechanosensitive AT1-B2 Receptor Heteromers. | ||
Cancer Res Transfer of Damaged Mitochondria from Cancer Cells to Cancer-Associated Fibroblasts Promotes Tyrosine Kinase Inhibitor Tolerance in EGFR-Mutant Lung Cancer. | ||
J Extracell Vesicles Umbilical Cord-Mesenchymal Stromal Cell-Derived Extracellular Vesicles Target the Liver to Improve Neurovascular Health in Type 2 Diabetes With Non-Alcoholic Fatty Liver Disease | ||
Nat Commun Single cell transcriptomic analyses implicate an immunosuppressive tumor microenvironment in pancreatic cancer liver metastasis | ||
Nat Commun Single-cell analysis reveals fibroblast heterogeneity and myeloid-derived adipocyte progenitors in murine skin wounds. | ||
Nat Commun Single-cell RNA sequencing highlights the role of inflammatory cancer-associated fibroblasts in bladder urothelial carcinoma. |
