CoraLite® Plus 488-conjugated RGS5 Polyclonal antibody
RGS5 Polyclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
Cat no : CL488-11590
Synonyms
Validation Data Gallery
Tested Applications
Positive IF/ICC detected in | HT-1080 cells |
Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-11590 targets RGS5 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | RGS5 fusion protein Ag2196 |
Full Name | regulator of G-protein signaling 5 |
Calculated Molecular Weight | 181 aa, 21 kDa |
Observed Molecular Weight | 42 kDa |
GenBank Accession Number | BC030059 |
Gene Symbol | RGS5 |
Gene ID (NCBI) | 8490 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RGS5 inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. It binds to G(i)-alpha and G(o)-alpha, but not to G(s)-alpha. The 42kd band recognized by this antibody is a RGS5-RGS5 dimer. (Pubmed:17762159)
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 RGS5 antibody CL488-11590 | Download protocol |
FC protocol for CL Plus 488 RGS5 antibody CL488-11590 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |