Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, HT-1080 cells, NIH/3T3 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human tonsillitis tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, NIH/3T3 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19856-1-AP targets PCM1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13929 Product name: Recombinant human PCM1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-254 aa of BC000453 Sequence: MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTSPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYM Predict reactive species |
| Full Name | pericentriolar material 1 |
| Calculated Molecular Weight | 2024 aa, 228 kDa |
| Observed Molecular Weight | 260-280 kDa |
| GenBank Accession Number | BC000453 |
| Gene Symbol | PCM1 |
| Gene ID (NCBI) | 5108 |
| RRID | AB_2878616 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15154 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Pericentriolar material 1 (PCM1) is a component of centriolar satellites, which are electron dense granules scattered around centrosomes (PMID: 10579718). PCM1 is required for centrosome assembly and function. It acts as a scaffold to target several proteins to the centrosome in a dynein motor-dependent manner and regulate microtubular dynamics (PMID:18762586; 12403812). It is also involved in the biogenesis of cilia (PMID: 18772192). Chromosomal aberrations involving PCM1 gene are associated with papillary thyroid carcinomas and a variety of hematological malignancies, including atypical chronic myeloid leukemia and T-cell lymphoma. (PMID: 10980597; 16091753; 15805263; 16424865).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PCM1 antibody 19856-1-AP | Download protocol |
| IHC protocol for PCM1 antibody 19856-1-AP | Download protocol |
| WB protocol for PCM1 antibody 19856-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PCM1 Polyclonal Antibody (Cat# 19856-1-AP) has 36 citations spanning journals including Science, Nature Communications, Circulation, EMBO Journal, Cell Reports, Developmental Cell, Journal of Cell Biology, EMBO Reports, eLife, and FASEB Journal. Research fields covered include centrosome biology, ciliogenesis, cell division, cardiomyopathy, atherosclerosis, cancer (breast, renal, thyroid), osteoclast function, and neuromuscular physiology. Cited applications include WB, IF, IHC, and IP, with reactivity in human, mouse, and rat.
| Species | Application | Title |
|---|---|---|
Circulation Jmjd4 Facilitates Pkm2 Degradation in Cardiomyocytes and Is Protective Against Dilated Cardiomyopathy | ||
Nat Commun Lipid droplets sequester palmitic acid to disrupt endothelial ciliation and exacerbate atherosclerosis in male mice | ||
Cardiovasc Diabetol Diastolic dysfunction in a pre-clinical model of diabetes is associated with changes in the cardiac non-myocyte cellular composition. |
Reviews
What customers say
Both reviewers gave 5-star ratings. One user confirmed the antibody recognized a protein at the expected molecular weight in Western Blot. Another user reported excellent and reliable performance in Immunofluorescence on primary fibroblasts at a 1:200 dilution, specifically noting compatibility with PFA fixation and methanol permeabilization. Overall, customers find this antibody specific, reliable, and well-suited for both WB and IF applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Boyan (Verified Customer) (11-25-2024) | recognized a protein at the expected size
|
Julie (Verified Customer) (01-06-2020) | This antibody works great and reliably with PFA fix & methanol permeabilization.
|
